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SP_H6_Halo_M21K_F86L_KDEL_pBABEpu

Plasmid

#183691

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(M21K_F86L)

Use

Retroviral

Mutation

M21K_F86L in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET30_Halotag_K73T_Jdomain

Plasmid

#183693

Purpose

Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)-J domain

Expression

Bacterial

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-CteIF4E_35-250-del183-202_AF

Plasmid

#148721

Purpose

Bacterial Expression of CteIF4E_35-250-del183-202

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_35-250-del183-202

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-CteIF4E_40-250-del183-202_AF

Plasmid

#148723

Purpose

Bacterial Expression of CteIF4E_40-250-del183-202

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_40-250-del183-202

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CteIF4E_35-250-del183-202_AF

Plasmid

#148725

Purpose

Bacterial Expression of CteIF4E_35-250-del183-202

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_35-250-del183-202

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-CteIF4G_996-1033-YLLtoAAA-GB1_AF

Plasmid

#148706

Purpose

Bacterial Expression of CteIF4G_966-1033-YLLtoAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4G_966-1033-YLLtoAAA

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-CtPAN2_1-457_Y

Plasmid

#148094

Purpose

Bacterial Expression of CtPAN2_1-457

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

CtPAN2_1-457

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-CtPAN2opt_1-490_V

Plasmid

#147787

Purpose

Bacterial Expression of CtPAN2opt_1-490

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

CtPAN2opt_1-490

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-CtPAN3opt_171-566_V

Plasmid

#147789

Purpose

Bacterial Expression of CtPAN3opt_171-566

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

CtPAN3opt_171-566

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

Plasmid

#185679

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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OM14-2EGFP-UG74-ter

Plasmid

#184758

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

OM14 (OM14 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2EGFP-ter-NAT

Plasmid

#184764

Purpose

Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CIT1-2EGFP-ter-URA3

Plasmid

#184767

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CIT1 (CIT1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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ACH1-2EGFP-ter-URA3

Plasmid

#184769

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ACH1 (ACH1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AFG3-2EGFP-ter-URA3

Plasmid

#184770

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

AFG3 (AFG3 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SYS1-2EGFP-ter-URA3

Plasmid

#184771

Purpose

Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SYS1 (SYS1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2Katushka-ter-Hygromycin

Plasmid

#184772

Purpose

Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

2Katushka

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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PKC1-2GFP-ter-TRP1

Plasmid

#184773

Purpose

Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PKC1 (PKC1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P40

Plasmid

#188057

Purpose

Expression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P40

Expression

Mammalian

Promoter

CMV promotor

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P50

Plasmid

#188058

Purpose

Expression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P50

Expression

Mammalian

Promoter

CMV promotor

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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