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Plasmid#153979PurposeDimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1bDepositorInsertmRNA decapping enzyme 1b (DCP1B Human)
UseTagspAcGFP-C1ExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_KanR neo
Plasmid#167885PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry
Plasmid#167902PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
human-GFAP-EGFP
Plasmid#169473PurposeWe generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.DepositorInsertEGFP-hGFAP (GFAP Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-GpNLuc
Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects..DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HiBiT-CD63
Plasmid#162591PurposeN-terminally HiBiT-tagged human CD63 under CAG promoterDepositorInserthuman CD63 (CD63 Human, human, Synthetic)
UseTagsHiBiTExpressionMammalianMutationPromoterCAG promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-OgNLuc
Plasmid#70186PurposeConstiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-mNG-NL
Plasmid#169106PurposeExpresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - mNeonGreen - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Sec61β
Plasmid#174466PurposeExpress EGFP with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertSirius with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-BeYDV-Rep-RepA-9
Plasmid#197715PurposePhytobrick (MoClo) Level 0 PartDepositorInsertBeYDV Rep/RepA proteins: native ORFs
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ch-gelsolN
Plasmid#37262DepositorInsertgelsolin (GSN Human)
UseTagsmCherryExpressionMammalianMutationdeleted amino acids 380-731PromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1
Plasmid#202010PurposeMoclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBIDepositorInsertNbGP41P-sfGFP-VP64-GB1
UseSynthetic Biology; Moclo level 1 vectorTagsGB1 and sfGFPExpressionMutationPromoterCmYLCVAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
PERK 2: PERK.K618A.9E10.pCDNA
Plasmid#21815DepositorInsertPERK (K618A) (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationkinase dead K618A mutantPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-Sec16S
Plasmid#15775DepositorInsertSec16S (SEC16B Human)
UseTagsmEGFPExpressionMammalianMutationN/APromoterAvailable sinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2
Plasmid#109020Purposestable lentiviral expression of cDNADepositorInsertATL2 (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-NbGP41-sfGFP-VP64-GB1-J9
Plasmid#202007PurposeMoClo level 0 NbGP41-sfGFP-VP64-GB1 coding sequence moduleDepositorInsertNbGP41-sfGFP-VP64-GB1
UseSynthetic Biology; Moclo cloning vectorTagsGB1 and sfGFPExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_mcherry
Plasmid#167886PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_vinculin
Plasmid#65691PurposeExpresses Vinculin-CNL in mammalian cellsDepositorInsertvinculin (Vcl Mouse)
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNeonGreen-LEHD-NanoLuc
Plasmid#98289Purposecaspase-9 BRET SensorDepositorInsertmNeonGreen-LEHD-NanoLuc
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_CBF-GLuc_L3-L2
Plasmid#113868PurposeSingle pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.DepositorInsertsCBF (notch dependent promoter)
GLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only