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pSMART-ala12

Plasmid

#87173

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proC

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pPJ169(JPUB_006769)

Plasmid

#83835

Purpose

Carries NEW operon 5 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste3340
Kuste3350

Promoter

op5_3350_for and op5_BsaI_3340_for

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPJ170(JPUB_006771)

Plasmid

#83833

Purpose

Carries NEW operon 6 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste3346
Kuste3348
Kuste3349

Promoter

op6_3348_for, op6_3349_for, and op6_BsmBI_3346_fo…

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pET24a_CDP-CHase

Plasmid

#73161

Purpose

Expresses in E. coli, Bacillus cereus NP_831800 CDP-CHase

Depositor

Maurice Bessman, Sandra Gabelli

Article

Duong-Ly et al J Bacteriol. 2011 Jul;193(13):3175-85. d

Insert

pET24a

Expression

Bacterial

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
FCIV1-GR SS287AA

Plasmid

#72703

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

SS287AA

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
DVK_FG

Plasmid

#66070

Purpose

MoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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DVK_GH

Plasmid

#66071

Purpose

MoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-mTmod3-1_163_domain

Plasmid

#70754

Purpose

mouse Tropomodulin3, 1-163 domain for bacterial expression

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 1-163 AA fragment (Tmod3 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pfMDH_Triple

Plasmid

#67733

Purpose

Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed histidine 303 to alanine, arginine 373 to…

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCGS652

Plasmid

#62220

Purpose

Mammalian expression TALEN vector with N152, C+18 truncations

Depositor

Daniel Voytas

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Type

Empty backbone

Use

TALEN

Tags

AcV5 and SV40 NLS

Expression

Mammalian

Promoter

miniCaggs

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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