We narrowed to 4,221 results for: 256

1. PX458_AHR_2

   Plasmid

   #101077

   Purpose

   Encodes gRNA for 3' target of human AHR

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against AHR (AHR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. STARR-seq luciferase validation vector_SCP1_empty

   Plasmid

   #99299

   Purpose

   Luciferase validation vector; empty vector without insert to determine baseline

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. GA-DEVD-GB-NES

   Plasmid

   #61020

   Purpose

   green ddFP based caspase-3 biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A and ddGFP B
   

   Use

   Tags

   HindIII and NES sequence LALKLAGLDIGS placed afte…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET28a His6Flag2AUF1(p37)

   Plasmid

   #135939

   Purpose

   Expresses AUF1p37 in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
   

   Use

   Tags

   Flag2 and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR221-BNT162b2ΔUTR

   Plasmid

   #171214

   Purpose

   Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

   Depositor

   Liang Wei Wang

   Article

   Low et al medRxiv 2021.04.27.21256151

   Insert

   BNT162b2ΔUTR
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Kozak sequence changed from CCCGCCACC to GGCTGCAC…

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETSUMO2_Rhodoplanes-bGSDM

   Plasmid

   #212129

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Rhodoplanes bGSDM
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETSUMO2_Bacteroidetes-bGSDM

   Plasmid

   #212124

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Bacteroidetes bGSDM
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETSUMO2_Runella-bGSDM

   Plasmid

   #212123

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Runella bGSDM
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETSUMO2_Vitiosangium-bGSDM

   Plasmid

   #212127

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Vitiosangium bGSDM
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETSUMO2_Gillisia-bGSDM

    Plasmid

    #212128

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Gillisia bGSDM
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a His6Flag2AUF1(p45)

    Plasmid

    #135942

    Purpose

    Expresses AUF1p45 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. HS-EB3-GFP

    Plasmid

    #105948

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRE-Tight-FLAG-CYLD-WT

    Plasmid

    #60030

    Purpose

    WT CYLD under control of Tet promoter with N-terminal FLAG tag

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

    Plasmid

    #167268

    Purpose

    Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HOB
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid

    #207148

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBa-FRB-3myc-Rab5a

    Plasmid

    #64209

    Purpose

    Rab5a with N-terminal FRB and triple myc tags

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Rab5a (Rab5a Mouse)
    

    Use

    Tags

    3myc and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    chicken Beta Actin

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)_UGControl

    Plasmid

    #133875

    Purpose

    Expresses UGControl in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

    Insert

    UtrCH (UTRN Human)
    

    Use

    Tags

    flexible linker - mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJ3 SHP2 E76A

    Plasmid

    #8321

    Depositor

    Ben Neel

    Article

    Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E76A (activated mutant)

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. plKO.1-DNAJC5-ShRNA

    Plasmid

    #205729

    Purpose

    Knockdown of DNAJC5

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    shRNA targeting DNAJC5 (DNAJC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFLAG W96A-GLTP

    Plasmid

    #170741

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    W96A

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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