We narrowed to 2,107 results for: Endog
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Plasmid#200380PurposeTagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminalDepositorInsertspaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
ExpressionMammalianMutationNAAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE smFP-V5 donor ORF1
Plasmid#200381PurposeTagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminalDepositorInsertspaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
ExpressionMammalianMutationNAAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Cdc42-2G
Plasmid#68814PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)DepositorInsertCdc42 activation reporter
TagsHis and MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGATOR-DTA
Plasmid#64103PurposeTet inducible, Gateway adapted ROSA26 targeting Vector with DTADepositorInsertrTTA (Gt(ROSA)26Sor Synthetic)
UseMouse Targeting; Tet inducibleExpressionMammalianPromoterROSA26 endogenousAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Eef25UTR-renilla
Plasmid#38235DepositorInsertEef2 5'UTR / Renilla (Eef2 Mouse)
UseLuciferaseExpressionMammalianMutationEef2 5' UTR fused to renilla ORFPromoterEndogenous Eef2Available SinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Eef25UTR-TOPmut-renilla
Plasmid#38236DepositorInsertEef2_TOPm_5UTR/Renilla (Eef2 Mouse)
UseLuciferaseExpressionMammalianMutationThe first nucleotides of the 5' UTR were cha…PromoterEndogenous Eef2Available SinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAC-EHER
Plasmid#53262PurposeContains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coliDepositorInsertcrtE, idi, crtX, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-iSH2
Plasmid#66839PurposeExpression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.DepositorInsertiSH2
TagsmCherry-CRY2ExpressionMammalianPromoterCMVAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD/CMV/V5-DEST-Cdc42-2G
Plasmid#68812PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)DepositorInsertCdc42 activation reporter
UseAdenoviralTagsHis and MycExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-85b
Plasmid#53282PurposeContains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthaseDepositorInsertcrtE, crtY, crtI
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSGV Mouse Acceptor PGK NGFR
Plasmid#128482PurposeA TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9DepositorTypeEmpty backboneUseRetroviralPromoterMSCVAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorInsertHaloTag DKC1
TagsHaloTagExpressionMammalianPromoterCMVAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TC
Plasmid#213398Purposetranscriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to CitrineDepositorInsertFingR for endogenous PSD95, transcriptinally regulated
UseAAVTagsCitrineExpressionMammalianAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
T/brachyury(2A-Dre)
Plasmid#140761PurposeTargeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.DepositorInsertDre
UseMouse TargetingPromoterEndogenous T/brachyuryAvailable SinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-IC3-FLAG-Tev-ZZ
Plasmid#170311PurposePlasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.DepositorInsertOuter Dynein Arm Intermediate Chain 3
TagsFLAG, ZZAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BIRC3-NU
Plasmid#160698PurposeExpresses GFP-BIRC3 without the endogenous 3'UTR in mammalian cellsDepositorInsertGFP-BIRC3 (BIRC3 Human)
ExpressionMammalianAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai
Plasmid#202571PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
TagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…Available SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
TagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only