We narrowed to 18,525 results for: MUT

1. Dual opposing T7 promoters reporter plasmid

   Plasmid

   #156457

   Purpose

   A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Insert

   NeoR/KanR
   

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7 promoter + rpsL reporter plasmid

   Plasmid

   #156455

   Purpose

   A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   rpsL
   TcR
   

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   tac and tet

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX2TK-YAP-WW1-RRK

   Plasmid

   #104306

   Purpose

   bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated

   Depositor

   Marius Sudol

   Insert

   YAP WW1 domain (YAP1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…

   Promoter

   tac

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTwist-iS-methadoneSnFR-Golgi

   Plasmid

   #220865

   Purpose

   Mammalian cell expression for subcellular pharmacokinetics with Golgi targeting of iS-methadoneSnFR

   Depositor

   Henry Lester

   Article

   Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

   Insert

   iS-methadoneSnFR
   

   Expression

   Mammalian

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHHM.X513.iS-methadoneSnFR

   Plasmid

   #220861

   Purpose

   Bacterial expression of iS-methadoneSnFR for purification and in vitro assays.

   Depositor

   Henry Lester

   Article

   Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

   Insert

   iS-methadoneSnFR
   

   Tags

   His6-HA and Myc

   Expression

   Bacterial

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-SF3B1-K700E-reporter

   Plasmid

   #200318

   Purpose

   A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Insert

   BSD-2A-EGFPi
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPFO*

   Plasmid

   #120400

   Purpose

   Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

   Depositor

   Arun Radhakrishnan

   Article

   Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

   Insert

   Mutant form of Perfringolysin O
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Amino acids 1-27 are deleted, starts at amino aci…

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV Puro SCRIB P305L

   Plasmid

   #88887

   Purpose

   SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

   Depositor

   Senthil Muthuswamy

   Article

   Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

   Insert

   SCRIB (SCRIB Human)
   

   Use

   Retroviral

   Mutation

   Proline to Leucine mutation @ 305th amino acid

   Promoter

   MSCV promoter

   Available Since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/T0-eGFP-NFATC2IP D394R

   Plasmid

   #221436

   Purpose

   Mammalian expression vector of eGFP tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interaction

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   D394R (charge-reverting mutation for interaction …

   Promoter

   CMV

   Available Since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA5-FRT/T0-3xFLAG-NFATC2IP D394R

    Plasmid

    #221211

    Purpose

    Mammalian expression vector of triple Flag tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interaction

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    D394R (charge-reverting mutation for interaction …

    Promoter

    CMV

    Available Since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMtb MCM

    Plasmid

    #138296

    Purpose

    Expression of Mtb MCM small and large subunit

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Inserts

    mutA
    mutB
    

    Tags

    His tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    inserted TEV cleavage site between His-tag and m…

    Promoter

    T7

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PE5max-SF3B1-K700E

    Plasmid

    #200317

    Purpose

    Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Inserts

    K700E-ng1+pg2
    PEmax-2A-MLH1dn
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. c1GFP-W53XKir7.1

    Plasmid

    #107183

    Purpose

    Generates Nonsense mutant clone

    Depositor

    Bikash Pattnaik

    Article

    Pattnaik et al Hum Mutat. 2015 Jul;36(7):720-7. doi: 10

    Insert

    KCNJ13 (KCNJ13 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    W53X

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKIF1C_R169W-mCherry-FRB

    Plasmid

    #185984

    Purpose

    Mammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    mCherry and FRB

    Expression

    Mammalian

    Mutation

    R169W - mutation causing HSP (SPG58) and silent m…

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

15. EXT1- D164A-pLJC2-3XFLAG

    Plasmid

    #215828

    Purpose

    Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-XL4 ASXL1 (1-1304)

    Plasmid

    #74244

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Expression

    Mammalian

    Mutation

    Contains ASXL1 amino acids 1-1304

    Promoter

    CMV

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pLPC-S22A-Progerin

    Plasmid

    #69065

    Purpose

    encodes a Progerin mutant where serine 22 is mutated to an alanine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

    Insert

    Progerin (LMNA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Progerin with serine 22 mutated to alanine

    Promoter

    CMV

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCMV6-XL4 ASXL1 (1-479) 3x FLAG

    Plasmid

    #74262

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains ASXL1 amino acids 1-479

    Promoter

    5’LTR

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

    Plasmid

    #81022

    Purpose

    Cancer-associated ASXL1 truncation mutant in retroviral vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.Y591X truncation

    Promoter

    5’LTR

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pLPC-S22A-LaminA

    Plasmid

    #69066

    Purpose

    encodes a mutant lamin A where serine 22 is mutated to a an alanine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

    Insert

    LaminA (LMNA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    serine 22 mutated in alanine

    Promoter

    CMV

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only