We narrowed to 5,840 results for: pCas

1. pCAGGS-CIBN-EGFP-KRasCT

   Plasmid

   #178527

   Purpose

   Expresses CIBN-EGFP-KRasCT in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CIBN-EGFP-KRasCT
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS/XBB.1.5-RBD

   Plasmid

   #213038

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/XBB.1.5-RBD (S )
   

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-Flag-hsDicer (K70A)

   Plasmid

   #41589

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K70A

   Promoter

   CMV

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCAGGS-SARS2-S-FKO (C-flag)

   Plasmid

   #159364

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS-CoV-2)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Furin-cleavage site mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX551-miniCMV-SpCas9D10A nickase

   Plasmid

   #216735

   Purpose

   Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   T7 and mini-CMV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Gag-FKBP-Myc

   Plasmid

   #177909

   Purpose

   HIV Gag-FKBP-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   HIV Gag (gag )
   

   Tags

   FKBP12 and Myc tag

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Bmpr2ΔKinase gRNA resistant

   Plasmid

   #163411

   Purpose

   Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Expression

   Mammalian

   Mutation

   The kinase domain (202-500 aa) is deleted. gRNA-t…

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

   Plasmid

   #196195

   Purpose

   Editing human TSC2 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-GFP-PQRv3-RFP

   Plasmid

   #73951

   Purpose

   Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

   Depositor

   Brian Chen

   Article

   Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

   Inserts

   sfGFP (superfolder GFP)
   RFP
   

   Tags

   A residual PQR peptide wii be left at the C-termi…

   Expression

   Mammalian

   Promoter

   Chicken beta actin and Chicken beta actin (shared…

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-SRGAP1FBAR(516a.a.)-EGFP

    Plasmid

    #168505

    Purpose

    Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP1-FBAR (SRGAP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-516

    Promoter

    CAG

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-EGFP/RFP-int

    Plasmid

    #19818

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGS-PB1-AG(Y63L)-LOV2

    Plasmid

    #178876

    Purpose

    Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen(Y63L)-LOV2
    

    Expression

    Mammalian

    Mutation

    AzamiGreen: Y63L

    Promoter

    CAG

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCASP-SptP120-K55-HilA

    Plasmid

    #153329

    Purpose

    Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K55 DARPin
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCaUra3-CUbo-AID*-GFP

    Plasmid

    #212775

    Purpose

    C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-dSpCas9-HF1

    Plasmid

    #92115

    Purpose

    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-attBr-ZsGreen-attP

    Plasmid

    #51265

    Purpose

    Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    attBr-ZsGreen-attP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-MBP-ATG9-P834A

    Plasmid

    #188082

    Purpose

    Expresses human ATG9-P834A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    P834A

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-RaichuEV-Rac1(T17N)

    Plasmid

    #164902

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    T17N mutation in the Rac1 domain which make the s…

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gma-pCAMBIA-1301-STTM171

    Plasmid

    #84080

    Purpose

    Disturb the expression of miR171 in soybean

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR171
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid

    #191388

    Purpose

    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_hSLBP(56_108)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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