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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,672 results for: pCas

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Showing: 1721 - 1740 of 2672 results

  1. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Publication
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Tags
    T2A GFP
    Expression
    Insect
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

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  6. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

    Plasmid
    #224123
    Purpose
    Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7-mutant (Q20A_Y23A_Y24F_F35A)
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Q20A_Y23A_Y24F_F35A in La(SSB)1-194
    Promoter
    CMV
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    224123-plasmid-map-sequence-id-442589

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  10. pB–DualPE

    Plasmid
    #235161
    Purpose
    For plant prime editing including large DNA fragment editing in wheat plants or other monocotyledons
    Depositor
    Zong Yuan
    Publication
    Zhao et al Nat Plants. 2025 Feb;11(2):191-205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Csy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
    CmYLCV-epegRNA-CaMV poly(A) signal
    Use
    CRISPR
    Mutation
    Detailed in manuscript
    Available since
    Oct. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    235161-plasmid-map-sequence-id-488642

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  14. Cas9 NGFR

    Plasmid
    #155279
    Purpose
    Lentiviral Cas9 expression with NGFR marker
    Depositor
    Martin Carroll
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9
    Expression
    Mammalian
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155279-plasmid-map-sequence-id-309983

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  15. PX458-gR2A_ISO

    Plasmid
    #124808
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSPCas9
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124808-plasmid-map-sequence-id-244309

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  17. cBEST2

    Plasmid
    #234658
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Publication
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    kasO*17tss and kasOp*
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234658-plasmid-map-sequence-id-492748

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  18. pX330_ATP1A1_G7

    Plasmid
    #173204
    Purpose
    Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G7 sgRNA + SpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173204-plasmid-map-sequence-id-339520

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  19. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  20. pSLQ10625

    Plasmid
    #239268
    Purpose
    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in primary mouse cortical neurons
    Depositor
    Stanley Qi
    Publication
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-dPspCas13b-EGFP-ABI
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    CAG
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239268-plasmid-map-sequence-id-474742

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Showing: 1721 - 1740 of 2672 results