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Plasmid#166150PurposeExpression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coliDepositorArticleInsertDDX5ΔPrD(1-535)
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationΔPrD(1-535)PromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP
Plasmid#166151PurposeExpression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coliDepositorArticleInsertDDX5ΔIDR(1-483)
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationΔIDR(1-483)PromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCri-H6-TEV-GRAB
Plasmid#128498PurposeExpresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.DepositorInsertGRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
UseTagsHis tag (6x)ExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCri-H6-TEV-GRAB-STREP
Plasmid#128499PurposeExpresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.DepositorInsertGRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
UseTagsHis tag (6x) and Strep tag (2x)ExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cultivarium POSSUM Toolkit
Plasmid Kit#1000000234PurposeThe POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.DepositorAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM3-IRES_BFP
Plasmid#179960PurposeExpression vector for human IFITM3. Co-expresses BFP controlled by an IRES element.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM1
Plasmid#179955PurposeExpression vector for human IFITM1.DepositorInsertIFITM1 (IFITM1 Human)
UseTagsExpressionMammalianMutationP13APromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
UseTagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM2
Plasmid#179956PurposeExpression vector for human IFITM2.DepositorInsertIFITM2 (IFITM2 Human)
UseTagsExpressionMammalianMutationA33V, T41M, V121IPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA216-CoV2-RBD-beta variant
Plasmid#194598PurposeMammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)DepositorInsertCoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-6xHis-hSMSr-ΔSAMD
Plasmid#236225PurposeExpress N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cellsDepositorInsertSAMD8 (SAMD8 Human)
UseTagsHexa histidine tag (6xHis-tag) and enterokinase s…ExpressionInsectMutationsterile alpha motif domain at N-terminal (1-231 b…PromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMVP-SEC24C-G220C
Plasmid#231560PurposepMVP expression vector for human SEC24C (G220C mutation, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)DepositorInsertSEC24C (G220C) (SEC24C Human)
UseLentiviralTagsV5ExpressionMammalianMutationG220C, silent PAM mutations (G67 (GGG>GGA) and…PromoterCMVAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMVP-SEC24C-S107F
Plasmid#231561PurposepMVP expression vector for human SEC24C (S107F mutation, closesd, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)DepositorInsertSEC24C (S107F) (SEC24C Human)
UseLentiviralTagsV5ExpressionMammalianMutationS107F, silent PAM mutations (G67 (GGG>GGA) and…PromoterCMVAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE FL NAP1 STOP C' EGFP IRES Blast
Plasmid#179102PurposeExpresses untagged full length NAP1DepositorInsertNak associated protein 1 (AZI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-N'-FLAG-HA-NAP1S318A
Plasmid#195410PurposeExpresses FLAG-HA tagged human NAP1 with S318A point mutationDepositorInsertNak associated protein 1 (AZI2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationSerine 318 turned to AlaninePromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP
Plasmid#216480Purposelentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excisionDepositorInsertsUseCre/Lox and LentiviralTagsExpressionMutationS66A, S89A, S117A, S311APromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP
Plasmid#216479Purposelentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excisionDepositorInsertsUseCre/Lox and LentiviralTagsExpressionMutationPromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM1-IRES_eGFP
Plasmid#179958PurposeExpression vector for human IFITM1. Co-expresses GFP controlled by an IRES element.DepositorInsertIFITM1 (IFITM1 Human)
UseTagsExpressionMammalianMutationP13APromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM2-IRES_eGFP
Plasmid#179959PurposeExpression vector for human IFITM2. Co-expresses GFP controlled by an IRES element.DepositorInsertIFITM2 (IFITM2 Human)
UseTagsExpressionMammalianMutationA33V, T41M, V121IPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
UseTags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2PromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only