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We narrowed to 17,597 results for: ERG

Showing: 2611 - 2640 of 17597 results

  1. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  6. pORTMAGE-3

    Plasmid
    #72678
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72678-plasmid-map-sequence-id-198909

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  7. pSL1425 (pSPIN, pCDF backbone)

    Plasmid
    #160735
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    160735-plasmid-map-sequence-id-317231

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  11. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid
    #127368
    Purpose
    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127368-plasmid-map-sequence-id-246345

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  12. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

    Plasmid
    #154827
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    TagBFP
    HygR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154827-plasmid-map-sequence-id-306202

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  14. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid
    #127370
    Purpose
    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127370-plasmid-map-sequence-id-246351

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  16. pORTMAGE-4

    Plasmid
    #72679
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72679-plasmid-map-sequence-id-198908

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  18. pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

    Plasmid
    #172508
    Purpose
    Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172508-plasmid-map-sequence-id-344931

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  19. pAnc80L65

    Plasmid
    #68837
    Purpose
    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details
    Depositor
    Luk Vandenberghe
    Article
    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1
    Insert
    Ancestral AAV Capsid Anc80L65
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Rep2
    Available since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    68837-plasmid-map-sequence-id-121219

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  20. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid
    #127354
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and mVenus
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127354-plasmid-map-sequence-id-246338

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  22. pORTMAGE-2

    Plasmid
    #72677
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    pL promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72677-plasmid-map-sequence-id-457485

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  24. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid
    #127366
    Purpose
    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127366-plasmid-map-sequence-id-246342

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  25. pGGD_MiniTurboID-YFP

    Plasmid
    #222434
    Purpose
    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.
    Depositor
    Dominique Bergmann
    Article
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222434-plasmid-map-sequence-id-440831

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  27. pGGB_YFP-MiniTurboID

    Plasmid
    #222433
    Purpose
    Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.
    Depositor
    Dominique Bergmann
    Article
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222433-plasmid-map-sequence-id-440826

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Showing: 2611 - 2640 of 17597 results