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pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128344

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb3 (Chrnb3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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Halo-tagged Sox17FNV

Plasmid

#206391

Purpose

Expresses Halo-tagged mouse SOX17FNV in mammalian cells, for single-molecule tracking.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Sox17FNV (Sox17 Mouse)

Tags

HaloTag

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mBrn2-3xflag

Plasmid

#206390

Purpose

Expresses 3xflag fused mouse BRN2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-dt/CBA-EGFP-R106bT

Plasmid

#163921

Purpose

Serves as control to pLV-PGK-dt/CBA-EGFP-106bT.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

dtomato and EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

Added 4-tandem reverse orientation-mir-106b-targe…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128341

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna6 (Chrna6 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB L154A/H250Y

Plasmid

#108491

Purpose

expression of EGFP-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFB-His-SKP1

Plasmid

#228368

Purpose

express human SKP1 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

SKP1 (SKP1 Human)

Tags

His

Expression

Insect

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-HaloDA1.0-IRES-EGFP-CAAX

Plasmid

#234410

Purpose

Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

Insert

GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX2

Plasmid

#206388

Purpose

Expresses human SOX2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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GBX

Plasmid

#64123

Purpose

a modified episomal (EBNA1/OriP) vector expressing human eGFP and BCL-xL genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

GFP-2A-BCL2L1 (BCL2 Human)

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Ai65(RCFL-tdT) targeting vector

Plasmid

#61577

Purpose

Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

pCAG-FSF-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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MBX

Plasmid

#64122

Purpose

a modified episomal (EBNA1/OriP) vector expressing human BCL2L1 (BCL-xL) gene

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

BCL2L1 (BCL2L1 Human)

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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AAV pmSyn1-EBFP-Cre

Plasmid

#51507

Purpose

Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV5

Insert

EBFP-Cre

Use

AAV

Promoter

pmSyn1

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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TIGRE docking site targeting vector

Plasmid

#61580

Purpose

Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Type

Empty backbone

Use

Mouse Targeting

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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Ai66(RCRL-tdT) targeting vector

Plasmid

#61578

Purpose

Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

CAG-RSR-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iFlpV

Plasmid

#140137

Purpose

can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iFlpV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-GST-FBXO22

Plasmid

#228367

Purpose

express human FBXO22 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXO22 (FBXO22 Human)

Tags

GST

Expression

Insect

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65449

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

tetO

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hKLF4

Plasmid

#206386

Purpose

Expresses human KLF4 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Ai95(RCL-GCaMP6f) targeting vector

Plasmid

#61579

Purpose

Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

CAG-LSL-GCaMP6-fast

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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