We narrowed to 11,503 results for: NSI

1. Bxb1-GT-Dox-AIO-TetOn_ETV2_Down-Tandem

   Plasmid

   #241393

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ETV2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-ETV2; rtTA-T2A-mTagBFP2 (ETV2 Human)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE; CAG

   Available since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+8-cyp-33e1(C295A, C439A)-flag-tev-halo-his

   Plasmid

   #227158

   Purpose

   For T-REX experiments of cyp-33e1 C295A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   cyp-33e1 (cyp-33E1 Nematode)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 295 and cysteine 439 to alanine

   Promoter

   CMV and SP6

   Available since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+8-CYP2A6(C439A)-flag-tev-halo-his

   Plasmid

   #227161

   Purpose

   For T-REX experiments of CYP2A6 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   CYP2A6 (CYP2A6 Human)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 439 to alanine

   Promoter

   CMV and SP6

   Available since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his

   Plasmid

   #227163

   Purpose

   For T-REX experiments of CYP2A6 C82A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   CYP2A6 (CYP2A6 Human)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 82 and cysteine 439 to alanine

   Promoter

   CMV and SP6

   Available since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDF_AtRaf1/Raf2/BSD2

   Plasmid

   #229511

   Purpose

   Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,BSD2

   Depositor

   Laura Gunn

   Article

   Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

   Inserts

   Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
   Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
   BSD2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N terminus chloroplast transit peptide truncation

   Promoter

   T7

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. hTln1-R12DD-SCLS

   Plasmid

   #191443

   Purpose

   expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397

   Depositor

   Ben Goult

   Article

   Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.

   Insert

   Human Talin1 R12DD (TLN1 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   deletion between residues K2375 and A2397

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet

   Plasmid

   #209411

   Purpose

   Expression of wild-type adult isoform of human SCN8A.

   Depositor

   Al George

   Article

   Vanoye et al JCI Insight. 2024 May 21;9(12):e177530.

   Insert

   SCN8A Variant 3, stabilized (SCN8A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Modifed human HSPA5 intron 4 inserted after bp237…

   Promoter

   CMV

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4 3xFLAG Halo G-Linker nsp1

   Plasmid

   #175426

   Purpose

   Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   G-linker nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   G-linker in place of the RNase destabilization do…

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA4 3xFLAG Halo R119A K120A nsp1

   Plasmid

   #175428

   Purpose

   Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 R119A K120A
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants R119A K120A

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4 3xFLAG Halo E36A E37A nsp1

    Plasmid

    #175429

    Purpose

    Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 E36A E37A
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants E36A E37A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO-BcRF1-ORF24-2xStrep

    Plasmid

    #138454

    Purpose

    Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1-ORF24 (BcRF1, ORF24 EBV/KSHV)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    BcRF1 amino acids 1-178 fused to ORF24 amino acid…

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO-UL87-ORF24-2xStrep

    Plasmid

    #138455

    Purpose

    Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    UL87-ORF24 (UL87, ORF24 HCMV/KSHV)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    UL87 amino acids 1-248 fused to ORF24 amino acids…

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid

    #153445

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid

    #153444

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-AAV2-hSyn-MerMAID1-Citrine

    Plasmid

    #126520

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID1
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mutation

    Promoter

    human synapsin

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO-ORF66 C423A-2xStrep

    Plasmid

    #131111

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 423 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO-ORF66 C399A-2xStrep

    Plasmid

    #131112

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 399 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO-ORF66 C393A-2xStrep

    Plasmid

    #131113

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 393 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO-ORF66 C355A-2xStrep

    Plasmid

    #131114

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 355 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-ORF66 C344A-2xStrep

    Plasmid

    #131115

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 344 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge