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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,768 results for: ins/1000

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Showing: 1741 - 1760 of 6768 results

  1. HSPA5A

    Plasmid
    #42447
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSPA5A (HSPA5 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. pcDNA_DiCre2_59.F2

    Plasmid
    #60209
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Synthetic, Bacteriophage P1, Human)
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 19-59 o…
    Promoter
    CMV
    Available Since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60209-plasmid-map-sequence-id-96295

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  3. pDBD-R

    Plasmid
    #50571
    Purpose
    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    Expression
    Yeast
    Mutation
    M1R (B-gal)
    Promoter
    Gal1
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. GFP Cav2.2 HA D665A pcDNA3

    Plasmid
    #206113
    Purpose
    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional
    Depositor
    Annette Dolphin
    Insert
    cacna1b (CACNA1B Rabbit)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    D665A
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206113-plasmid-map-sequence-id-415392

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  6. DIRAS2

    Plasmid
    #38819
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DIRAS2 (diras2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pcDNA3.1/hpa5

    Plasmid
    #59972
    Purpose
    integrin a5 wild-type headpiece expression (a5 beta-propeller and a5 thigh domain)
    Depositor
    Timothy Springer
    Insert
    human integrin a5 headpiece (ITGA5 Human)
    Tags
    acid coiled-coil and StrepII tag
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. R777-E006 Hs.AKT3-nostop

    Plasmid
    #70290
    Purpose
    Gateway ORF clone of human AKT3 [NM_005465.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    AKT 3 (AKT3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70290-plasmid-map-sequence-id-125739

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  9. PSD-95_Y12E-pTagRFP

    Plasmid
    #52672
    Purpose
    expression of TagRFP-tagged PSD-95_Y12E
    Depositor
    Johannes Hell
    Insert
    PSD-95 (Dlg4 Rat)
    Tags
    tagRFP
    Expression
    Mammalian
    Mutation
    Y12E
    Promoter
    CMV
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52672-plasmid-map-sequence-id-81956

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  10. LASP1A

    Plasmid
    #42440
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    LASP1A (LASP1 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pMal-Clu-tail

    Plasmid
    #215396
    Purpose
    Expression of a C-terminal fusion protein of E. coli MBP with human Clusterin residues 204-238.
    Depositor
    Franz-Ulrich Hartl
    Insert
    MBP-Clu(204-238) (CLU Human)
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    215396-plasmid-map-sequence-id-430531

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  13. GR-SmBiT Vector (TK)

    Plasmid
    #237159
    Purpose
    Express GR-SmBiT Fusion Protein in Mammalian Cells under a TK promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    GR (NR3C1 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    TK
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237159-plasmid-map-sequence-id-474669

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  14. LgBiT-GR Vector (TK)

    Plasmid
    #237160
    Purpose
    Express LgBiT-GR Fusion Protein in Mammalian Cells under a TK promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    GR (NR3C1 Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    TK
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237160-plasmid-map-sequence-id-473354

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  15. pHUE-Clu-H7-GFP

    Plasmid
    #232021
    Purpose
    Expression of a His6-Ubiquitin-Clu(241-262)-eGFP fusion protein
    Depositor
    Franz-Ulrich Hartl
    Insert
    Clu(241-262)-eGFP (CLU Human)
    Tags
    His6-Ubiquitin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    232021-plasmid-map-sequence-id-470415

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  16. pB-T-PAF-Clu AB1

    Plasmid
    #215393
    Purpose
    High expression of the Clusterin(V26D,M34W,Q37E) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Expression
    Mammalian
    Mutation
    p.Val26Asp, p.Met34Trp, p.Arg37Glu
    Promoter
    tetO
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215393-plasmid-map-sequence-id-431821

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  17. pB-T-PAF-Clu TL3

    Plasmid
    #215383
    Purpose
    High expression of the Clusterin(L229S,M230S,F232S,Y235S) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Expression
    Mammalian
    Mutation
    p.Leu229Ser, p.Met230Ser, p.Phe232Ser, p.Tyr235Ser
    Promoter
    tetO
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215383-plasmid-map-sequence-id-431808

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  18. pB-T-PAF-Clu CC3

    Plasmid
    #215388
    Purpose
    High expression of the Clusterin(V369K,A373T,T376K) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Expression
    Mammalian
    Mutation
    p.Val369Lys, p.Ala373Thr, p.Thr376Lys
    Promoter
    tetO
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215388-plasmid-map-sequence-id-440344

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  19. Rab11A[8-175]-His-C Q70L

    Plasmid
    #228042
    Purpose
    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).
    Depositor
    Wade Harper
    Insert
    Rab11A[8-175]-His-C Q70L (RAB11A Human)
    Expression
    Bacterial
    Mutation
    Q70L
    Available Since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    228042-plasmid-map-sequence-id-450388

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  20. pCIneo-lambdaN-HA-HsAGO2-P593GA623F_AG

    Plasmid
    #148743
    Purpose
    Mammalian Expression of HsAGO2-P593GA623F
    Depositor
    Elisa Izaurralde
    Insert
    HsAGO2-P593GA623F (AGO2 Human)
    Expression
    Mammalian
    Mutation
    Three silent mutations and sequence differs in th…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148743-plasmid-map-sequence-id-291191

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Showing: 1741 - 1760 of 6768 results