164,909 results

1. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Oliver Weichenrieder, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / Lac

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6.2_mCherry_Grx1_roGFP2

   Plasmid

   #155045

   Purpose

   Fluorescent redox reporter

   Depositor

   Vanessa LaPointe

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AASDHPPT

   Plasmid

   #39192

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AASDHPPT (AASDHPPT Human)
   

   Use

   Tags

   6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pMpGWB102

   Plasmid

   #68556

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28GSTlinker-LIC

   Plasmid

   #119970

   Purpose

   Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. CD-MPR-GFP-RUSH

   Plasmid

   #202797

   Purpose

   RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

   Depositor

   Juan Bonifacino

   Article

   Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

   Insert

   M6PR (M6PR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed 40 K (AAA) with R (AGA)

   Promoter

   CMV

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-myc-Atg7

   Plasmid

   #24921

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   Atg7 (ATG7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. HypoxCR

   Plasmid

   #59946

   Purpose

   A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

   Depositor

   Anne Le

   Article

   Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

   Inserts

   GFP-Pest
   mCherry-Geminin
   

   Use

   Lentiviral

   Tags

   FLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and min CMV

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT59

    Plasmid

    #223431

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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