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We narrowed to 18,888 results for: REV

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Showing: 4401 - 4450 of 18888 results

  8. PKA1280

    Plasmid
    #239370
    Purpose
    His-Las17 (300-422) pTOPO 6xHis-Las17 (300-422) as a BamH1-HindIII fragment
    Depositor
    Kathryn Ayscough
    Insert
    Las17 (amino acids 300-422)
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239370-plasmid-map-sequence-id-478749

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  9. Sc Pol3-FLAG(Ec)-pRSF405/GAL

    Plasmid
    #239198
    Purpose
    Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)
    Depositor
    Michael O'Donnell
    Insert
    Pol3 (POL3 Budding Yeast)
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    E. coli codon bias (synthetic gene), but same ami…
    Available Since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239198-plasmid-map-sequence-id-474564

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  10. CPH3737_GST-TEV-Spt6p-tSH2-1223-1453

    Plasmid
    #227087
    Purpose
    Bacterial expression of K1355A/K1435A double-mutant GST-tagged Spt6 tSH2 domain (1223-1453)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    K1355A, K1435A
    Promoter
    T7
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    227087-plasmid-map-sequence-id-470399

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  11. pLS406

    Plasmid
    #231078
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231078-plasmid-map-sequence-id-460262

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  12. pLS405

    Plasmid
    #231077
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker LEU2
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231077-plasmid-map-sequence-id-459803

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  13. pLS404

    Plasmid
    #231076
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker TRP1
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231076-plasmid-map-sequence-id-459800

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  14. pLS408

    Plasmid
    #231079
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231079-plasmid-map-sequence-id-459799

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  15. pLS403

    Plasmid
    #231075
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker HIS3
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231075-plasmid-map-sequence-id-459792

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  19. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

    Plasmid
    #227086
    Purpose
    Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227086-plasmid-map-sequence-id-451101

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  20. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

    Plasmid
    #227084
    Purpose
    Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    STREPii-FLAG and SV40 NLS 1
    Expression
    Yeast
    Promoter
    Gal1-10
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227084-plasmid-map-sequence-id-451056

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  21. CUP1-∆ssCPY*-mScarletI

    Plasmid
    #221157
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221157-plasmid-map-sequence-id-450959

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  22. CUP1-∆ssCPY*-mNeonGreen

    Plasmid
    #221156
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221156-plasmid-map-sequence-id-450957

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  23. pX458-tRNA-SpCas9

    Plasmid
    #195183
    Purpose
    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.
    Depositor
    Stephanie Roessler
    Insert
    Cas9
    Use
    CRISPR
    Promoter
    tRNA-Gln
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    195183-plasmid-map-sequence-id-423143

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  24. HCP4

    Plasmid
    #166106
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166106-plasmid-map-sequence-id-330381

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  25. pNSU299_RPL39

    Plasmid
    #166078
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166078-plasmid-map-sequence-id-330349

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  26. HCP6

    Plasmid
    #166108
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166108-plasmid-map-sequence-id-330318

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  27. pNSU299_YDR344C

    Plasmid
    #166072
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166072-plasmid-map-sequence-id-329603

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  28. pNSU299_TDH3

    Plasmid
    #166084
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of TDH3 (TDH3 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166084-plasmid-map-sequence-id-329123

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  30. pBSIIKS_HIS3_I

    Plasmid
    #109062
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Expression
    Bacterial and Yeast
    Promoter
    HIS3
    Available Since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109062-plasmid-map-sequence-id-209541

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Showing: 4401 - 4450 of 18888 results