We narrowed to 14,236 results for: CAN
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Plasmid#114675PurposeThis construct was used to express mouse Otop2 in Xenopus laevis oocytesDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only
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pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)
Plasmid#181788PurposeExpresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDragon-empty
Plasmid#155018PurposeEmpty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.DepositorTypeEmpty backboneUseMouse TargetingAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)
Plasmid#181790PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCH6 - pBait-ChiX
Plasmid#174662Purposearabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.DepositorInsertChiX
Tags1xMS2 hairpinExpressionBacterialPromoterpBadAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV Aquamarine-shadowG tandem
Plasmid#157774PurposeProduces a fusion between Aquamarine and shadowG. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneTagsAquamarine and shadowGExpressionMammalianAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_VSVgSS-diCR2
Plasmid#207324PurposeExpression of secreted diCR2-10his competitor protein which can inhibit VSV-G mediated infectionDepositorInsertdiCR2 (LDLR Human)
ExpressionMammalianMutationfragment of LDL-R dimerizedPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
shNTC
Plasmid#73549PurposeNon-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).DepositorInsertshNTC
UseRetroviralExpressionMammalianPromoterLTRAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_peroxisome_C1
Plasmid#85064PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
TagsmScarlet-HExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBT344_pattB-pCA-GFP-pA-FRT5
Plasmid#52550Purposefor Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing miceDepositorInsertsPhiC31o attB (full length)
GFP
ExpressionMammalianAvailable SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)
Plasmid#181787PurposeExpresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTnsC, ShCas12k-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP
Plasmid#103815PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV Aquamarine-superYFP tandem
Plasmid#157776PurposeProduces a fusion between Aquamarine and superYFP. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneTagsAquamarine and superYFPExpressionMammalianAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-mCherry
Plasmid#103816PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLLX13
Plasmid#79825PurposeYeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vectorDepositorTypeEmpty backboneUseYeast and bacteria shuttle vector, contains origi…Available SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ
Plasmid#192380PurposeTo test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F3bbs kan NNNTGGGCGk
Plasmid#18756DepositorInsert3 tandem zinc fingers
ExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…Available SinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F1bbs kan GCGTGGNNN
Plasmid#18753DepositorInsert3 tandem zinc fingers
ExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…Available SinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEN-R2-mCsfGFP-L3
Plasmid#178602PurposeIt can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.DepositorInsertTandem fluorescent protein timers (tFTs)
UseEntry vector for gateway cloningTagsmCherry superfolder GFPAvailable SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only