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pLenti6/V5-DEST-SPAG5

Plasmid

#31217

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

SPAG5 (SPAG5 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Contains a one nucleotide deletion at C-term that…

Available Since

Aug. 19, 2011

Availability

Academic Institutions and Nonprofits only
FLAG-IKKbeta (S177E,S181E)-pcw107

Plasmid

#64609

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

IKBKB

Use

Lentiviral

Tags

FLAG

Mutation

S177E, S181E

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

Plasmid

#155307

Purpose

Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP

Use

Lentiviral

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Rheb (Q64L)-pcw107

Plasmid

#64607

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Mutation

Q64L

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

Plasmid

#236242

Purpose

Lentiviral expression of SpCas9

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SpCas9

Use

Lentiviral

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-Mkk7-JNK2 fusion-pcw107

Plasmid

#64618

Purpose

Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Map2k7 linker MAPK9

Use

Lentiviral

Tags

FLAG

Mutation

mouse-human fusion

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-HSF1

Plasmid

#31210

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

HSF1 (HSF1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-AmCyan

Plasmid

#138481

Purpose

For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

AmCyan

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MDFIC

Plasmid

#207835

Purpose

Tagged MDFIC for western blotting and imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

MDFIC (MDFIC Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 WT-Puro

Plasmid

#123319

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-FASCIN

Plasmid

#31207

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

FASCIN (FSCN1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-mEGFP-reHaloTagF

Plasmid

#200190

Purpose

Tom20 with reversible HaloTagF variant for fluorescent labeling

Depositor

Jacob Piehler

Article

Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

Insert

Tom20 (TOMM20 Human)

Tags

mEGFP and reHaloTagF

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

Plasmid

#216477

Purpose

lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

dsRed
tagBFP

Use

Cre/Lox and Lentiviral

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-MCM7

Plasmid

#31212

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

MCM7 (MCM7 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-HMGB1

Plasmid

#31208

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

HMGB1 (HMGB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pHAGE-GTF2IRD1__ALK

Plasmid

#116821

Purpose

Lentiviral expression of GTF2IRD1__ALK fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GTF2IRD1__ALK

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-GW-A

Plasmid

#138480

Purpose

For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-PIK3CA-pcw107

Plasmid

#64605

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

PIK3CA (PIK3CA Bovine)

Use

Lentiviral

Tags

FLAG and myristoylated

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pHAGE-ALDH5A1__MAP3K5

Plasmid

#116807

Purpose

Lentiviral expression of ALDH5A1__MAP3K5 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALDH5A1__MAP3K5

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51-GOLPH3

Plasmid

#21690

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Invitrogen gateway destination vector

Tags

6xHIS and V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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