We narrowed to 5,803 results for: SPI

1. SARS2nsp3_MacroD_ECBio2

   Plasmid

   #234388

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp3_SARS2
   

   Use

   Tags

   LVPRGSSSKGGYGLNDIFEAQKIEWHE and MHHHHHHSSGVDLGTEN…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. nsp14_EC_Bio1

   Plasmid

   #234376

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp14_SARS2
   

   Use

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SARS2nsp3_MacroD_EC

   Plasmid

   #234389

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp3_SARS2
   

   Use

   Tags

   MHHHHHHSSGVDLGTENLYFQSM

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. phCMV-229E-D15

   Plasmid

   #188908

   Purpose

   express truncated 229E spike for pseudovirus production

   Depositor

   David Nemazee

   Insert

   229E spike (S 229E)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c-terminal 15aa deletion

   Promoter

   CMV

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.3_SARS2_XBB.1.5

   Plasmid

   #196585

   Purpose

   expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (XBB.1.5)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G252V, F486P on the basis of XBB

   Promoter

   CMV

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

   Plasmid

   #160629

   Purpose

   TU for the expression of LbCas12a

   Depositor

   Diego Orzaez

   Insert

   AtUBQ10-:LbCpf1(Cas12a):Tnos
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   UBQ10

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET24a MDH_AQUAR

   Plasmid

   #204239

   Purpose

   Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDH_AQUAR
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p112A1NE

   Plasmid

   #85983

   Purpose

   For construction of cDNA expression library.

   Depositor

   Wolf Frommer

   Article

   Riesmeier et al EMBO J. 1992 Dec;11(13):4705-13.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Adh1

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-EgC*

   Plasmid

   #20378

   Depositor

   Andrew Lee

   Article

   Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

   Insert

   Eglin C wt*
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F10W

   Promoter

   Available since

   April 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAIP4_6WEY

    Plasmid

    #234234

    Purpose

    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pAIP4_5HIH

    Plasmid

    #234205

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pAIP4_2H2Z

    Plasmid

    #234147

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. paGFP

    Plasmid

    #18697

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    photoactivatable GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    CMV

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MS-1 magA

    Plasmid

    #21751

    Depositor

    Elliot Meyerowitz

    Article

    Bertani et al Gene. 2001 Feb 21. 264(2):257-63.

    Insert

    magA (magA M. magnetotacticum)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pPS904

    Plasmid

    #8850

    Depositor

    Pamela Silver

    Article

    Kahana et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

    Insert

    NUF2-sGFP (NUF2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

17. SARS2_HisSUMOTEV-nsp7/nsp8

    Plasmid

    #234405

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Inserts

    Nsp7_SARS2
    Nsp8_SARS2
    

    Use

    Tags

    His6-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NSP12:4393-5324_Nbio_Chis_BV

    Plasmid

    #234377

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp12_SARS2
    

    Use

    Baculovirus expresssion

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NSP13:5325-5925_BVnAvi

    Plasmid

    #234375

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp13_SARS2
    

    Use

    Baculovirus expresssion

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SARS1_NSP13_BV_NBIO-TEV_CTHROM-his

    Plasmid

    #234382

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp13_SARS
    

    Use

    Baculovirus expresssion

    Tags

    LVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge