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Plasmid#118218PurposeFor broad or variegated gal4 inducible expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamK2-tTA
Plasmid#210731PurposeAAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)DepositorInserttetracycline-controlled transactivator (tTA)
UseAAVTagsExpressionMutationPromoterCamK2Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF19
Plasmid#52644Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF19
UseTagsExpressionMammalianMutationT328D/T330D mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMEH280 NLS dTomato 3x high affinity MS2 loops
Plasmid#168211PurposeMammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CENP-B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45214DepositorInsertCENP-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromotert7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-FAID
Plasmid#127885PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID taggingDepositorInsertGFP-Puro-H3F-AID (PPP2CA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)
Plasmid#92401PurposeConstitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLIB-TetR-KRAB-IRES-BLAS
Plasmid#55820Purposeretroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistanceDepositorInsertTetR-KRAB
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPI278
Plasmid#113227Purposeextrachromosomal expression vector, expression of H2B-GFPDepositorInsertH2B
UseDictyostelium discoideum expression vector, extra…TagsGFPExpressionMutationPromoteract15Available sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTYF-sGFAP-IRES-tdTomato
Plasmid#166920PurposeEmpty plasmid backbone; includes IRES-tdTomato and sGFAP promoterDepositorTypeEmpty backboneUseLentiviralTagstdTomatoExpressionMammalianMutationPromotersGFAPAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-EcPPK1
Plasmid#159279PurposeExpresses a HA-tagged E. coli PPK1 in mammalian cell linesDepositorInsertHA-tagged Polyphosphate Kinase 1
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
Plasmid#31477DepositorInsertSFFV HA NLS Y2 T2A mTagBFP
UseLentiviralTagsHA and NLSExpressionMutationF13Y + S111Y on I-AniI = Y2 "reo" (res…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVCERC-5 Pvan-dcas9 (Sth3)
Plasmid#133319Purposeexpresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedTagsExpressionMutationPromotervanillateAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAN147
Plasmid#129202Purposeyeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backboneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM178
Plasmid#61270PurposeAlso referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterpLtetO, native, native (respectively)Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV LDTS-LIRmut
Plasmid#189001Purposelipophagy-inducing adaptor protein, negative controlDepositorInsertLDTS-LIRmut
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEx-insertion-tet
Plasmid#172211PurposeFor genetic insertion in tetracyclin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseGenetic insertion in prevotella copriTagsExpressionMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAL119
Plasmid#21909DepositorInserthCMV promoter expression cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSV(X) C/EBPdelta
Plasmid#13394DepositorInsertC/EBPdelta (Cebpd Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMito-dCherry-FRB
Plasmid#186573PurposeExpression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cellsDepositorInsertDark MitoTrap
UseTagsExpressionMammalianMutationMutation of "K70N" to render mCherry no…PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only