161,383 results

1. pCMV-PE6e

   Plasmid

   #207855

   Purpose

   Mammalian expression of PE6e prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6e
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Cas9(H840A, K918A, K775R)

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP1

   Plasmid

   #79873

   Purpose

   Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

   Depositor

   Carol Gross, Jason Peters, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   xylA

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-TLR6-YFP

   Plasmid

   #13020

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 6 (TLR6 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pADsacA

   Plasmid

   #158649

   Purpose

   Constitutive transcription of sacA crRNA and GFP

   Depositor

   Wenjing Cui

   Insert

   sacA crRNA
   

   Use

   CRISPR and Synthetic Biology; Shuttle vector baci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

   Plasmid

   #50460

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   EF1a

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-dLight1.3b

   Plasmid

   #125560

   Purpose

   For expression of the genetically encoded dopamine sensor dLight1.3b

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   dLight1.3b
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB506

   Plasmid

   #74848

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDonor14-N7HELIX-KanR

   Plasmid

   #190663

   Purpose

   Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   NA

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-hARFGAP1-HA

   Plasmid

   #206409

   Purpose

   Mammalian expression of hARFGAP1

   Depositor

   Kazuhisa Nakayama, Hye-Won Shin

   Article

   Saitoh et al J Biol Chem. 2009 May 15;284(20):13948-1

   Insert

   ARFGAP1 (ARFGAP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENN.AAV.CamKII0.4.eGFP.WPRE.rBG

    Plasmid

    #105541

    Purpose

    AAV expression of EGFP from CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1 and AAV5

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamkII

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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