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pET_RGG-RFP-RGG

Plasmid

#124944

Purpose

Exoression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His and mCherry

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF4a

Plasmid

#67497

Purpose

Expression of shRNA targeting human ARF4a

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF4 shRNA (ARF4 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pOpLacI:mini35ss (GB0534)

Plasmid

#68179

Purpose

Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xOpLacI:mini35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBTK599s

Plasmid

#110601

Purpose

BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP dropout

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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5mCUC1 pGreenII0129

Plasmid

#12067

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Use

Plant transformation via agrobacterium

Mutation

miR-resistant CUC1. Made by introducing five sile…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pROSA26-G19

Plasmid

#21711

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 G19 (Gt(ROSA)26Sor Mouse)

Expression

Bacterial

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pET28a-SpyTag002 DA-MBP

Plasmid

#102832

Purpose

Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002 DA-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Reactive aspartic acid has been mutated to an ala…

Promoter

T7

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-mWasabi-G4

Plasmid

#164168

Purpose

Expresses mWasabi under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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phNFL-P440L

Plasmid

#132596

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Mutation

1319C>T; Pro440Leu

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Venus-Cdc20 R132A (1768)

Plasmid

#26059

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Tags

Venus

Expression

Mammalian

Mutation

Mutation in the Mad2 binding site, R132A

Available Since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only