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Plasmid#90501PurposepSIN - EF-1alpha - MTS tHO1 - P2A - MTS - tPCYA, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase, under EF-1alpha promoterDepositorInsertmito-tHO1 and mito-tPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-234
Plasmid#90507PurposepSIN - EF-1alpha - MTS sHO1 - P2A - MTS - sPCYA, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoterDepositorInsertmito-sHO1, mito-sPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-240
Plasmid#90510PurposepSIN - EF-1alpha - Cyto -sFd - P2A - Cyto - sFNR, vector encoding for cytoplasmic-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoterDepositorInsertcyto-sFD, cyto-sFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pPKm-300
Plasmid#104626PurposeExpresses pSIN - EF-1alpha - MTS - tFd, vector encoding mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd), under EF-1alpha promoterDepositorInserttFd
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1alphaAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Lenti puro EF2 LUC-2A-Dendra2
Plasmid#129427Purposeexpression of LUC and Dendra2 reporters under EF2 promoterDepositorInsertLUC Dendra2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
DH-BB(5'SIN;TE12ORF)
Plasmid#72309PurposeDefective Helper to produce neurotropic, propagation-incompetent Sindbis virusDepositorInsertDH-BB(5'SIN;TE12ORF)
UseSindbis virus helper constructTagsExpressionMutationPromoterAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL001
Plasmid#26122DepositorInserttTR-KRAB
UseLentiviralTagsIRES-PuroExpressionMutationPromoterAvailable sinceJune 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)
Plasmid Kit#1000000175PurposePlasmids and entry vectors for diverse fluorescent sensors of physiological parameters in cells.DepositorAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZapLck biosensor
Plasmid#131584PurposeLck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRETDepositorInsertZapLck biosensor
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP
Plasmid#114106Purposefusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)
Plasmid#114101Purposefusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-NanoLuc-VChR1-EYFP
Plasmid#114112Purposefusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertNanoLuc; Volvox channelrhodopsin 1; EYFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114099Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114104Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)
Plasmid#114100Purposefusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-EYFP-Kv
Plasmid#89256PurposeChannelrhodopsin localized to neuronal soma via a Kv2.1 targeting motifDepositorInsertChannelrhodopsin
UseAAVTagsEYFPExpressionMammalianMutationH134RPromotersynapsinAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-Kv-P2A-H2b-mRuby2
Plasmid#89570PurposeChannelrhodopsin localized to neuronal soma with simultaneous nuclear labelingDepositorInsertsChannelrhodopsin
Histone 2B
UseAAVTagsmRuby2ExpressionMammalianMutationH134RPromotersynapsinAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUiLMO2W
Plasmid#129480Purpose3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin CAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-iLMO2
Plasmid#129474PurposeMammalian expression vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 ChR2 ET-TC 2A tDimer
Plasmid#101361PurposeHigh efficiency channelrhodopsin, neuron specific promoter, red fluorescent proteinDepositorInsertsChannelrhodopsin-2
synaptophysin-red fluorescent protein
UseAAVTagsExpressionMammalianMutationE123T , T159CPromoterhuman SynapsinAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)
Plasmid#72889Purposefusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, Volvox channelrhodopsin 1, EYFP
Volvox channelrhodopsin 1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CaMKIIa::iLMO2
Plasmid#129481PurposeAAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoterDepositorInsertiLMO2
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
Plasmid#66779PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertshbeRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-double floxed-eNpHR-EYFP-WPRE-pA
Plasmid#20949PurposeCre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibitionDepositorHas ServiceAAV9Inserthalorhodopsin
UseAAVTagsEYFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_RapACR_T111C_N248Q-EYFP
Plasmid#119197PurposeMammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248QDepositorInsertAnion channelrhodopsin RapACR fast mutant T111C_N248Q
UseTagsEYFPExpressionMammalianMutationchanged threonine 111 to cysteine and asparagine …PromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ChR2-Venus
Plasmid#15753PurposeExpresses ChR2-Venus for neuronal excitation.DepositorInsertChannelrhodopsin2-Venus
UseTagsVenusExpressionMammalianMutationPromoterCAGAvailable sinceOct. 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
GtCCR2_EYFP_pcDNA3.1
Plasmid#159121PurposeThis plasmid encodes GtCCR2 rhodopsin domainDepositorInsertGtCCR2
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GtCCR1_EYFP_pcDNA3.1
Plasmid#159120PurposeThis plasmid encodes GtCCR1 rhodopsin domainDepositorInsertGtCCR1
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
VV001: WT Arch-3 in pET28b
Plasmid#58487PurposeExpresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3 (with C-terminal His tag)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only