Addgene

We narrowed to 10,138 results for: SEC

Showing: 161 - 180 of 10138 results

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pLenti-X1-Neo-HA-SEC62

Plasmid

#139865

Purpose

Lentiviral expression of HA-tagged human SEC62

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

SEC62 (SEC62 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-hATG9A

Plasmid

#58995

Purpose

Expresses SECFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

Venus

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-hATG16A1

Plasmid

#58994

Purpose

Expresses SECFP-hATG16A1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)

Use

Retroviral

Tags

SECFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-mGFP-Sec23B

Plasmid

#66598

Purpose

Expresses Sec23B as a fusion with mGFP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23B (SEC23B Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Sec61β

Plasmid

#174466

Purpose

Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_V5-APEX2-Sec61B

Plasmid

#83412

Purpose

Expressed N-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-Sec61B (SEC61B Human, Synthetic)

Use

Tags

APEX2 and V5

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHLSec2-irisin-his

Plasmid

#122729

Purpose

Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Bovine, Rat, Human, Mouse)

Use

Tags

his and signal sequence for secretion

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-shR

Plasmid

#208358

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations introduced to render shRNA …

Promoter

CMV

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac211-Sec31-yoHalo

Plasmid

#115423

Purpose

Express Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec31-yoHalo

Use

Tags

yoHaloTag

Expression

Yeast

Mutation

Promoter

Endogenous

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSecTag-T210A-ATX

Plasmid

#17840

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

T210A-ATX (ENPP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pMRX-IP-SECFP-Galectin3

Plasmid

#64150

Purpose

Expression in mammalian cells (production of retrovirus vector)

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Use

Retroviral

Tags

SECFP

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pBABE-SSeCKs T751A

Plasmid

#107142

Purpose

full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs T751A (Akap12 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed threonine 751 to alanine

Promoter

gag

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v1 IQSEC1 WT

Plasmid

#162016

Purpose

Expression of IQSEC1 splice variant 1

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v2 IQSEC1 WT

Plasmid

#162017

Purpose

Expression of IQSEC1 splice variant 2

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRiboI-Sec-APEX2m

Plasmid

#176845

Purpose

Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-Sec-APEX2m

Use

Tags

Expression

Bacterial

Mutation

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-SECFP

Plasmid

#175838

Purpose

Express SECFP with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with nucleus-targeting sequence

Use

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v3 IQSEC1 WT

Plasmid

#162018

Purpose

Expression of IQSEC1 splice variant 3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v4 IQSEC1 WT

Plasmid

#162019

Purpose

Expression of IQSEC1 splice variant 4

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_SEC13:1-322

Plasmid

#211229

Purpose

Mammalian expression of full-length human SEC13. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

SEC13:M1-Q322 (SEC13 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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