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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 418 results for: asns

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Showing: 161 - 180 of 418 results

  1. pJK382

    Plasmid
    #71574
    Purpose
    Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)
    Depositor
    T. J. Kappock
    Article
    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91
    Insert
    citrate synthase
    Expression
    Bacterial
    Mutation
    changes aspartate-317 to asparagine; silent mutat…
    Promoter
    recA
    Available Since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71574-plasmid-map-sequence-id-130942

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  10. SARS-CoV spike all5KO mutant

    Plasmid
    #231921
    Purpose
    For expression of SARS-CoV spike protein with glycan deletion at N1080, N1116, N1140, N1155, N1176 by implementing Asn-to-Gln mutation
    Depositor
    Sriram Neelamegham
    Article
    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10
    Insert
    spike all5KO (S SARS-CoV)
    Tags
    C9 and CD5 signal peptide
    Expression
    Mammalian
    Mutation
    N1080Q, N1116Q, N1140Q, N1155Q, N1176Q
    Promoter
    CMV
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231921-plasmid-map-sequence-id-459043

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  11. MERS-CoV spike all7KO mutant

    Plasmid
    #231924
    Purpose
    For expression of MERS-CoV spike protein with glycan deletion at N1176, N1213, N1225, N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutation
    Depositor
    Sriram Neelamegham
    Article
    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10
    Insert
    spike all7KO (S MERS-CoV)
    Expression
    Mammalian
    Mutation
    N1176Q, N1213Q, N1225Q, N1241Q, N1256Q, N1277Q, N…
    Promoter
    CMV
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231924-plasmid-map-sequence-id-459044

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  18. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    Huanting Liu, James Naismith
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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Showing: 161 - 180 of 418 results