We narrowed to 294 results for: cas9 f cas9 r

1. pLD-puro-Cc-CR-PARK7H126A-VA

   Plasmid

   #115190

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLD-puro-Cc-CR-PARK7E163K-VA

   Plasmid

   #115191

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAi14-luc-MC

   Plasmid

   #87113

   Purpose

   minicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   Luciferase-SV40pA
   

   Use

   CRISPR and Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-mTubb3

   Plasmid

   #87116

   Purpose

   AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAi14-GFPNLS-MC

   Plasmid

   #87114

   Purpose

   minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   GFPNLSPpA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   nEF

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Ai14-HITI

   Plasmid

   #87117

   Purpose

   AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Tubb3gRNA-mCherry

   Plasmid

   #87111

   Purpose

   mouse Tubb3 target gRNA expression plasmid with CAGmCherry

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   Mouse Tubb3 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60229

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60228

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60227

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-rMERTK-HITI

    Plasmid

    #87119

    Purpose

    Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Ai14-luc

    Plasmid

    #87118

    Purpose

    AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA
    

    Use

    AAV, CRISPR, and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GUCY2F gRNA (BRDN0001145143)

    Plasmid

    #77253

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GUCY2F

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GUCY2F (GUCY2F Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GUCY2F gRNA (BRDN0001146166)

    Plasmid

    #77255

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GUCY2F

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GUCY2F (GUCY2F Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GUCY2F gRNA (BRDN0001145320)

    Plasmid

    #77256

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GUCY2F

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GUCY2F (GUCY2F Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GUCY2F gRNA (BRDN0001147943)

    Plasmid

    #77254

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GUCY2F

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GUCY2F (GUCY2F Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHelp

    Plasmid

    #227583

    Purpose

    Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    guide RNA scaffold
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT25_GalL_BE3

    Plasmid

    #143736

    Purpose

    Expresses BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT42_GalL_BE-PAPAPA

    Plasmid

    #145035

    Purpose

    Expresses BE-PAPAPA in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-PAPAP
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT23_GalL_BE-PAPAP

    Plasmid

    #143486

    Purpose

    Expresses BE-PAPAP in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-PAPAP
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge