We narrowed to 7,171 results for: cas9 plasmid

1. pLV-TET2BlastR-Cas9-TagBFP

   Plasmid

   #236726

   Purpose

   Dox Inducible Cas-TagBFP expression

   Depositor

   David Bersten

   Insert

   Cas9-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP18-dCas9_O

   Plasmid

   #127028

   Purpose

   Basic Part O- ORF; Catalytically dead mutant of the Cas9 from Streptococcus pyogenes.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part dCas9_O
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-dCas9-LSD1-K661A-Hygro

   Plasmid

   #104414

   Purpose

   transient expression of dCas9-dLSD1 fusion protein

   Depositor

   Stephan Beck

   Insert

   dLSD1 (KDM1A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   IRATp970A0364D (Source Bioscience) has a P405H ch…

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-dCas9-BFP-KRAB

   Plasmid

   #221502

   Purpose

   CRISPRi machinery fish codon optimized

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   D10A and H839A point mutations

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-dTET1-H1672Y-D1674A-Hygro

   Plasmid

   #104413

   Purpose

   transient expression of dCas9-dTET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   dTET1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   H1672Y-D1674A

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-dCas9-JMJD2A-S288G-T289G-Hygro

   Plasmid

   #104418

   Purpose

   transient expression of dCas9-dJMJD2A fusion protein

   Depositor

   Stephan Beck

   Insert

   dJMJD2A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   S288G-T289G

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-dCas9-p300-S1396R-S1397R-Hygro

   Plasmid

   #104419

   Purpose

   transient expression of dCas9-dp300 fusion protein

   Depositor

   Stephan Beck

   Insert

   dp300
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   S1396R-S1397R

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PtPuc3_diaCas9_sgRNA

   Plasmid

   #109219

   Purpose

   Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

   Depositor

   Per Winge

   Article

   Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

   Inserts

   LHCF2 promoter
   diaCas9
   LHCF1 terminator
   U6 promoter
   sgRNA
   U6 3' region
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   LHCF1 terminator, LHCF2 promoter, U6 3' regi…

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9_nonPuro_OSS

   Plasmid

   #186652

   Purpose

   Cas9 plasmid to express the Cas9 in OSS cells

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Cas9 Expression Plasmid
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mPLD3-sgRNA-Cas9-mcherry

    Plasmid

    #199700

    Purpose

    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMST665_BB3_L 23_gRNAempty_cas9_BbsI

    Plasmid

    #90278

    Purpose

    BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    Cas9
    

    Use

    A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid

    #192663

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, D839A, H840A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid

    #121173

    Purpose

    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

    Depositor

    Timo Otonkoski

    Insert

    dCas9-VP192-T2A-EGFP
    

    Use

    CRISPR and Lentiviral; Inducible by doxycycline

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.3 CAS9 IRES-Dsred

    Plasmid

    #129444

    Purpose

    expression of FnCas9 and DsRed

    Depositor

    Oskar Laur

    Insert

    FnCas9 IRES-Dsred
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid

    #126881

    Purpose

    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid

    #126882

    Purpose

    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p425_Cas9_gRNA_LEU_1014a

    Plasmid

    #87407

    Purpose

    p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cas9_YTHDF2_sgRNA

    Plasmid

    #186673

    Purpose

    YTHDF2 sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9-sgRNA-RFP

    Plasmid

    #166005

    Purpose

    Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

    Depositor

    Martin Buck

    Article

    Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pISV-CRISPR/Cas9PGmUbi

    Plasmid

    #209189

    Purpose

    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection

    Depositor

    Christian Staehelin

    Inserts

    Cas9
    DsRed(deltaB)
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    missing BsaI site and plant-codon optimized with …

    Promoter

    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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