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Plasmid#194512PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertcLAP(EGFP)-P2A-NGFR
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag NGFR EGFP cLAP -0
Plasmid#194511PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertcLAP(EGFP)-P2A-NGFR
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag NGFR EGFP cLAP +1
Plasmid#194509PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertcLAP(EGFP)-P2A-NGFR
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag NGFR EGFP nLAP -1
Plasmid#194506PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertNGFR-P2A-nLAP(EGFP)
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag NGFR EGFP cLAP +0
Plasmid#194508PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertcLAP(EGFP)-P2A-NGFR
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag NGFR EGFP nLAP +1
Plasmid#194315PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertNGFR-P2A-nLAP(EGFP)
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag NGFR EGFP nLAP +0
Plasmid#194314PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertNGFR-P2A-nLAP(EGFP)
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCriprV2-sgRNA-CRY2-#1
Plasmid#189989PurposeLentiviral Cas9/sgRNA vector targeting C-terminus of hCRY2, works with Addgene 189983-189986DepositorInsertCryptochrome-2 (CRY2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-25xUPRE-minP-EGFP
Plasmid#159668PurposeEGFP reporter plasmid containing 25 tandem repeats of the the unfolded protein response element (UPRE)DepositorInsert25xUPRE-minP-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_ELAVL1
Plasmid#106106PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1DepositorInsertgRNA targeting ELAVL1 (ELAVL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_ELAVL2
Plasmid#106107PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL2DepositorInsertgRNA targeting ELAVL2 (ELAVL2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-PE2-P2A-BFP
Plasmid#231580PurposeExpresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA proteinDepositorInsertPE2-P2A-BFP
UseLentiviralTagsP2A-BFPExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDlg/pRRE-int-MS2M
Plasmid#166032PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertGag-Pol-MS2
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL-H1-ccdB-mEF1a-RiH
Plasmid#60601PurposeCloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.DepositorInsertsRed Fluorescent Protein
Hygromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgRNA targeting human SLC12A5 gene stop codon
Plasmid#158577PurposeFor generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInsertsgRNA targeting human SLC12A5 gene stop codon
UseCRISPRTagsCas9 and GFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-miniSdd7-BE4max-BlastR
Plasmid#204851PurposeFor cytosine base editing using miniSdd7 in mammalian cellsDepositorInsertminiSdd7-nSpCas9(D10A)-UGI
UseCRISPRTagsExpressionMammalianMutationD10A in SpCas9PromoterCMV, SV40, EM7Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-miniSdd6-BE4max-BlastR
Plasmid#204850PurposeFor cytosine base editing using miniSdd6 in mammalian cellsDepositorInsertminiSdd6-nSpCas9(D10A)-UGI
UseCRISPRTagsExpressionMammalianMutationD10A in SpCas9PromoterCMV, SV40, EM7Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-miniSdd3-BE4max-BlastR
Plasmid#204849PurposeFor cytosine base editing using miniSdd3 in mammalian cellsDepositorInsertminiSdd3-nSpCas9(D10A)-UGI
UseCRISPRTagsExpressionMammalianMutationD10A in SpCas9PromoterCMV, SV40, EM7Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S293A
Plasmid#115204PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only