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pSB1C3-pT7-sfGFP_RED20

Plasmid

#135173

Purpose

Expresses sfGFP recoded in the RED20 genetic code off the T7 promoter

Depositor

Drew Endy

Article

Calles et al Nucleic Acids Res. 2019 Sep 12. pii: 556

Insert

sfGFP_RED20

Use

Synthetic Biology

Mutation

Recoded ORF of sfGFP to only use one codon per am…

Promoter

taatacgactcactata

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

Plasmid

#232935

Purpose

Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

T2A-eGFP-SV40pA with Grb10 homology arms

Use

Mouse Targeting

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTuLys2CMR2

Plasmid

#53544

Purpose

Inhibition module. Constructed from the parent plasmids pLuxRI, pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 lysozyme by inducing AHL and LuxR. Chloramphenicol resistance

Depositor

Lingchong You

Article

Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

Insert

pLux

Use

Unspecified

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mCherry

Plasmid

#87664

Purpose

retroviral vector encoding cre dependent expression of membrane mCherry

Depositor

Oscar Marin

Article

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

Mem-mCherry

Use

Retroviral

Promoter

CAG

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBS xChordin

Plasmid

#24975

Depositor

Edward De Robertis

Article

Sasai et al Cell. 1994 Dec 2. 79(5):779-90.

Insert

Chordin (chrd.1.S Frog)

Expression

Bacterial

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO C1-APEX2

Plasmid

#129641

Purpose

Express ER-localized C1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

C1(1-29)-APEX2-GFP

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Pxyl-zapT-mScarlet-I

Plasmid

#207249

Purpose

Integrative plasmid for expressing ZapT-mScarlet-I in Caulobacter crescentus CB15N under Pxyl promoter

Depositor

Suliana Manley

Article

Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:

Insert

ZapT-mScarlet-I

Tags

mScarlet-I

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus cerberus

Plasmid

#24679

Depositor

Edward De Robertis

Article

Bouwmeester et al Nature. 1996 Aug 15. 382(6592):595-601.

Insert

Cerberus (cer1 Frog)

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-Crispr-V2-HMID.v1

Plasmid

#103062

Purpose

LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

Cas9 plus expressed GESTALT V1-V5 guide

Use

Lentiviral

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPVxRF3

Plasmid

#138385

Purpose

Venus knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

Venus

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMT-halo-HA-p2a-neoR

Plasmid

#161977

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GAlinker-HA-halo-HA-p2a-neoR

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPRxRF3

Plasmid

#138386

Purpose

TagRFP-T knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

TagRFP-T

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Neurod1

Plasmid

#74396

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurod1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Neurog1

Plasmid

#74395

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenin 1 (Neurog1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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XE9 Xwnt-8 CSKA

Plasmid

#16866

Depositor

Randall Moon

Article

Christian et al Genes Dev. 1993 Jan. 7(1):13-28.

Insert

Xwnt-8 (wnt8a.L Frog)

Use

Xenopus expression

Tags

myc?

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pGEMT-aid-p2a-GFP

Plasmid

#161976

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GSlinker-aid-GFP

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-GFP-p2a-aid

Plasmid

#161975

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GFP-aid-GSlinker

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-bovine-tau

Plasmid

#15864

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

bovine tau (MAPT )

Available Since

May 6, 2008

Availability

Academic Institutions and Nonprofits only