We narrowed to 365 results for: pme i

1. pFnCas12a_NT

   Plasmid

   #169838

   Purpose

   Expresses FnCas12a and guide RNA in Bacteria

   Depositor

   John van der Oost

   Article

   Mohanraju et al CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1

   Insert

   FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pXynA

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDragon-empty

   Plasmid

   #155018

   Purpose

   Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

   Depositor

   Alberto Rosello-Diez

   Article

   Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol1:uas-myc-act-map2k2b-2a-tdTomato

   Plasmid

   #104721

   Purpose

   UAS-driven expression of activated MEK

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   activated map2k2b (map2k2b Zebrafish)
   

   Use

   Zebrafish transposon

   Tags

   2A-tdTomato and myc tag

   Expression

   Mutation

   serines 221 and 225 to aspartic acid

   Promoter

   10xUAS

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS-Fv2Eflt4um200-2a-tdTomato

   Plasmid

   #67698

   Purpose

   Expression construct

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   Fv2E-flt4-2A-tdTomato (flt4 Zebrafish)
   

   Use

   Tags

   2A-tdTomato and HA

   Expression

   Mutation

   um200

   Promoter

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pUASTLOTattB_mCherry-CD8-vhhGFP

   Plasmid

   #163930

   Purpose

   Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pONSY-mCherry

   Plasmid

   #111874

   Purpose

   This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pONSY-Lifeact:mCherry

   Plasmid

   #111876

   Purpose

   This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Lifeact fused to mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pONSY-Venus

   Plasmid

   #111875

   Purpose

   This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Venus
   

   Use

   Capsaspora owczarzaki

   Tags

   Venus

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAct-GST-H3

   Plasmid

   #182842

   Purpose

   A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-HA-JMJD3

    Plasmid

    #24167

    Purpose

    Mammalian expression of human JMJD3 with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-HA-UTX

    Plasmid

    #24168

    Purpose

    Mammalian expression of human UTX with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

    Plasmid

    #67687

    Purpose

    UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    dusp6 (dusp6 Zebrafish)
    

    Use

    Tags

    6xmyc-NLS-tdTomato-2A

    Expression

    Mutation

    S159A, S196A

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pONSY-CoH2B:Venus

    Plasmid

    #111877

    Purpose

    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora Histone H2B (CoH2B) fused to Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJS646

    Plasmid

    #183175

    Purpose

    Y2H vector compatible with pJS647 PYR1 insert

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LjUBQ:MtGLV6:T35s

    Plasmid

    #164741

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr3g095180.1
    

    Use

    Tags

    Terminator 35S

    Expression

    Plant

    Mutation

    Promoter

    Lotus japonicus Ubiquitin

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LjUBQ:MtGLV2:T35s

    Plasmid

    #164740

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr5g012400.1
    

    Use

    Tags

    Terminator 35S

    Expression

    Plant

    Mutation

    Promoter

    Lotus japonicus Ubiquitin

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. aTY50_pPGK1_Pcad_IRES_PuroR_WPRE

    Plasmid

    #225348

    Purpose

    Lentiviral vector for Pcad expression

    Depositor

    Wendell Lim

    Article

    Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

    Insert

    Pcad (Cdh3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. aTY65_pTRE_Wnt3a_IRES_tagBFP2_WPRE

    Plasmid

    #225349

    Purpose

    Lentiviral vector for Wnt3a and BFP expression

    Depositor

    Wendell Lim

    Article

    Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

    Insert

    Wnt3a (Wnt3a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LjUBQ:MtGLV1:T35s

    Plasmid

    #164738

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr6g043230.1
    

    Use

    Tags

    Terminator 35S

    Expression

    Plant

    Mutation

    Promoter

    Lotus japonicus Ubiquitin

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LjUBQ:MtGLV9:T35s

    Plasmid

    #164742

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr4g116360.1
    

    Use

    Tags

    Terminator 35S

    Expression

    Plant

    Mutation

    Promoter

    Lotus japonicus Ubiquitin

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge