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pCAG-HA-rat Nrxn1beta AS4(-)

Plasmid

#58267

Purpose

Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Publication

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1beta AS4(-) (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMRX-IZ-ANXA11-mRuby3

Plasmid

#184894

Purpose

Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Publication

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA11 (ANXA11 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

R230C relative to NP_001148.1

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCri-13a

Plasmid

#61321

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Publication

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and MISTIC-tag

Expression

Bacterial

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pICH41414

Plasmid

#50337

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3-myc-MmUbc7/2776

Plasmid

#11488

Depositor

Allan Weissman

Publication

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MmUbc7 (Ube2g2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

n/a

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
Gal4BD-CRY2(535)

Plasmid

#75369

Purpose

Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

Depositor

Chandra Tucker

Publication

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Promoter

ADH1

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-AktAR2

Plasmid

#64933

Purpose

Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

Depositor

Jin Zhang

Publication

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LAMP1-AktAR2 (LAMP1 Budding Yeast, Synthetic, Human)

Tags

Cerulean3, Full-length LAMP1, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

Plasmid

#63169

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-FLAG"

Tags

N-myristoylation

Expression

Insect

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDDGFP2_leu2d_NRT1.1

Plasmid

#58332

Purpose

Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

Depositor

Simon Newstead

Publication

Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

Insert

NRT1.1 (NRT1.1 Mustard Weed)

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAS160

Plasmid

#194483

Purpose

synDHBR Cassette

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synDHBR Cassette

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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M72-IRES-tauLacZ LTNL TV

Plasmid

#15638

Depositor

Peter Mombaerts

Publication

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M72-IRES-tauLacZ LTNL TV

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
P3-IRES-tauLacZ LNL TV

Plasmid

#15642

Depositor

Peter Mombaerts

Publication

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

P3-IRES-tauLacZ LNL TV

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
P3-IRES-tauGFP LNL TV

Plasmid

#15643

Depositor

Peter Mombaerts

Publication

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

P3-IRES-tauGFP LNL TV

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold

Plasmid

#186981

Purpose

mammalian expression of Cas7-11 crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mature DR guide

Expression

Mammalian

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Septin 11 isoform III

Plasmid

#120319

Purpose

Septin 11 isoform III mammalian expression

Depositor

Angel de Blas

Publication

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform I

Plasmid

#120314

Purpose

Septin 11 isoform I mammalian expression

Depositor

Angel de Blas

Publication

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLX307/PTPN11 E76G

Plasmid

#140862

Purpose

expresses PTPN11 E76G

Depositor

Kimberly Stegmaier

Publication

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Expression

Mammalian

Mutation

E76G

Promoter

EF1a

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMYC BRGI aa597-1407

Plasmid

#19146

Depositor

Joan Massague

Publication

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Tags

Myc

Expression

Mammalian

Mutation

human BRG1 aa 597-1407 (PCR product)

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pJRH-1328 LV EF1a-CD20 IRES-EGFP

Plasmid

#201918

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD20

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28-His6-Ubc9

Plasmid

#133909

Purpose

Bacterial expression of His6-Ubc9

Depositor

Jeffrey Bode

Publication

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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