We narrowed to 5,652 results for: PHI-1

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Cav2.2 HA D122A pcDNA3

Plasmid

#206108

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCIG3 N

Plasmid

#132941

Purpose

MLV packaging plasmid for N-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

N-tropic MLV gag-pol

Use

Retroviral

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCIG3 B

Plasmid

#132942

Purpose

MLV packaging plasmid for B-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

B-tropic MLV gag-pol

Use

Retroviral

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pXGFPC-5 Pxyl-dcas9 (Sth3)

Plasmid

#133317

Purpose

expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus thermophilus #3)

Use

Unspecified

Promoter

xylose

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pVCERC-5 Pvan-dcas9 (Sth3)

Plasmid

#133319

Purpose

expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus thermophilus #3)

Use

Unspecified

Promoter

vanillate

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pcDNA3

Plasmid

#206069

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-IRES-let-7 8x

Plasmid

#20932

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

8x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Mutation

168bp IRES from 5'UTR of reaper gene in Dros…

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only
pAc5.1-PH-mEGFP-Cup isoform B (JM051)

Plasmid

#206451

Purpose

Cup expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Cup (cup Fly)

Tags

PH mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmCaf40_25-291_AE

Plasmid

#148619

Purpose

Bacterial Expression of DmCaf40_25-291

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmCaf40_25-291 (Rcd-1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-Y139DG146E_AE

Plasmid

#148589

Purpose

Insect Expression of DmCaf40-Y139DG146E

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmCaf40-Y139DG146E (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1

Plasmid

#21684

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Decapping protein 1 (Dcp1 Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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T1P/pUBQ10(mTurq->mScarlet)

Plasmid

#195945

Purpose

PhiC31 target with pUBQ10 switching from mTurq to mScarlet

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

tRBCS-mScarlet-attP_PhiC31-pUBQ10-attB_PhiC31-mTurq-tUBQ10

Expression

Plant

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGe1-V5His6_A

Plasmid

#145867

Purpose

Insect Expression of DmGe1

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

DmGe1 (Ge-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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KHBD00607

Plasmid

#39685

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG32788 (Crg-1 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00368

Plasmid

#34483

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4143 (mbf1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCFD3-frame_selector_1

Plasmid

#127554

Purpose

Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 1

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-white[coffee]

Plasmid

#84006

Purpose

Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4

Depositor

Phillip Zamore

Article

Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

Insert

A 2,080 bp fragment of the white[coffee] allele (w Fly)

Use

Unspecified

Mutation

A GC-to-AA mutation that creates a G589E missense…

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pWPT-PDX1

Plasmid

#12256

Depositor

Didier Trono

Article

Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

Insert

Pdx1 (PDX1 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pThermeleon

Plasmid

#86120

Purpose

Bandpass temperature-gated expression of mWasabi and mCherry reporters

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi
TcI38
mCherry
cI

Tags

6xHis

Expression

Bacterial

Mutation

A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

Promoter

pR/pL, pR/pL, pLacI, and pTlpA

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pX601‐MHP1‐miniABEmaxNG‐N2‐(GG)

Plasmid

#187067

Purpose

Gp41-1 Split N-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmaxNG-Gp41

Use

AAV and CRISPR

Expression

Mammalian

Promoter

MHP1

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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