We narrowed to 2,475 results for: T2A

1. pGH394

   Plasmid

   #230851

   Purpose

   Ef1a-rAPOBEC1-dCas9-T2A-BlastR-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   rAPOBEC1-dCas9
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGH398

   Plasmid

   #230854

   Purpose

   Ef1a-rAPOBEC1-n'Cas9-T2A-BlastR-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   rAPOBEC1-n'Cas9
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGH401

   Plasmid

   #230852

   Purpose

   Ef1a-rAPOBEC1-nCas9-T2A-BlastR-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   rAPOBEC1-nCas9
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-GfaABC1D-eGFP-2A-mCherry_D4H

   Plasmid

   #194878

   Purpose

   Ratiometric D4H sensor for in vivo astrocyte-specific cholesterol visualization

   Depositor

   Peter Scheiffele

   Article

   Czernecki et al bioRxiv 2022.11.16.516849

   Insert

   GFP-T2A-mCherry-D4H
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-GfaABC1D-eGFP-2A-mCherry_YDA

   Plasmid

   #194879

   Purpose

   Ratiometric YDA sensor for in vivo astrocyte-specific cholesterol visualization

   Depositor

   Peter Scheiffele

   Article

   Czernecki et al bioRxiv 2022.11.16.516849

   Insert

   GFP-T2A-mCherry-YDA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUltra-MDH1-ME1

   Plasmid

   #184465

   Purpose

   Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydrogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Lentiviral

   Tags

   fused to T2A

   Expression

   Mammalian

   Mutation

   deletion of stop codon

   Promoter

   UbC

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   tTRE, hPGK

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PS Intein

   Plasmid

   #242019

   Purpose

   Blue-light-induced reconstitution of split mCherry.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

   Insert

   PS Intein
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. eGFP L202 Reporter

   Plasmid

   #119129

   Purpose

   Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   mCherry and eGFP
   

   Use

   Lentiviral

   Mutation

   eGFP L202S

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p160Tol2-her4.3:switchCas9

    Plasmid

    #227680

    Purpose

    Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    loxP-dsRed-loxP-Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Promoter

    U6 and her4.3

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP L138 Reporter

    Plasmid

    #119130

    Purpose

    Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    mCherry and eGFP
    

    Use

    Lentiviral

    Mutation

    eGFP L138S

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMT-PTE2A

    Plasmid

    #111773

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

    Plasmid

    #227775

    Purpose

    Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    BFP-MCS
    mCherry-t2A-CreERT2
    

    Use

    Zebrafish expression

    Promoter

    Olactb and hsp70l

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPaint-sfGFP-3xP3-RFP

    Plasmid

    #127566

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sfGFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dimer cleaved

    Plasmid

    #198199

    Purpose

    FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mVenus-T2A-mTurquoise2
    

    Expression

    Mammalian

    Mutation

    A206K both

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn-DiO-eGFP-2A-mCherry_D4H

    Plasmid

    #194880

    Purpose

    Ratiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualization

    Depositor

    Peter Scheiffele

    Article

    Czernecki et al bioRxiv 2022.11.16.516849

    Insert

    GFP-T2A-mCherry-D4H
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. eGFP Y93H TC editing reporter

    Plasmid

    #198882

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Y93H

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBLO1811_Cas9_noNLS_human

    Plasmid

    #74490

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNW3

    Plasmid

    #53372

    Purpose

    Csy4 and Cas9 D10A nickase expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9n (D10A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Promoter

    Dmel Hsp70Bb

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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