We narrowed to 5,840 results for: pCas

1. pX330-Flag-eSpCas9-H840A

   Plasmid

   #80454

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9-Puro HLAG-2B-sgRNA

   Plasmid

   #182552

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2B-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCa4B2G-QUAS-DSCP-FLPo

   Plasmid

   #24359

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   QUAS-DSCP-FLPo
   

   Expression

   Insect

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. Osa-pCAMBIA-1301-STTM159

   Plasmid

   #84195

   Purpose

   Disturb the expression of miR159 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR159
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

   Plasmid

   #148855

   Purpose

   Mammalian Expression of HsAGO3-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO3-sgRNA (AGO3 S.pyogenes)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-SRGAP2A-FBAR-mRFP

   Plasmid

   #168502

   Purpose

   Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

   Depositor

   Franck Polleux

   Article

   Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

   Insert

   SRGAP2A-FBAR (SRGAP2 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-SpCas9_sgRNA-site2 (RTW448)

   Plasmid

   #160137

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)

   Plasmid

   #128171

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-AaCas12b(R911A)-2AeGFP

   Plasmid

   #121961

   Purpose

   Mammalian expression, Transcription regulation, deactivated AaCas12b

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   Catalytically deactivated AaCas12b
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   R911A

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX544 SpCas9(del1098-1368)

    Plasmid

    #58889

    Purpose

    Truncated form of SpCas9 without C' domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 1098-1368

    Available Since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-dHeFSpCas9-VP64-6xHis

    Plasmid

    #92119

    Purpose

    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-PB1-MontiRed-LOV2

    Plasmid

    #178872

    Purpose

    Mammalian expression of PB1-MontiRed-LOV2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-MontiRed-LOV2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAWp63-clone32 (Opti-SpCas9)

    Plasmid

    #131736

    Purpose

    Expresses Opti-SpCas9 in mammalian cells

    Depositor

    Alan Siu Lun Wong

    Article

    Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

    Insert

    Opti-SpCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    R661A+K1003H

    Promoter

    EFS

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Osa-pCAMBIA-1301-STTM394

    Plasmid

    #84204

    Purpose

    Disturb the expression of miR394 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR394
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCASP-SptP120-E3_5-HilA

    Plasmid

    #153326

    Purpose

    Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-E3_5 DARPin
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98976

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(N863A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCASP-SptP120-K27-HilA

    Plasmid

    #153328

    Purpose

    Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K27 DARPin
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCA-ATG-intron-tdT3Myc

    Plasmid

    #36883

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tdTomato-3Myc
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    beta-globin intron containing a loxP site inserte…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-RFP-miR-E1-1int

    Plasmid

    #19823

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Insert

    miRNA
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pBW809_pCAG-PV6-F3-mCherry-BGHpA

    Plasmid

    #87585

    Purpose

    PV6 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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