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kank2_pJet1.2

Plasmid

#121980

Purpose

for generating kank2 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank2 (LOC571549 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pN1-mCherry-NKD2

Plasmid

#176641

Purpose

Expresses NKD2-mCherry fusion protein in mammalian cells

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_EBFP2

Plasmid

#167873

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcold-6xHis-HRV3Csite-DmLoqsPB

Plasmid

#41094

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PB (loqs Fly)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179

Plasmid

#91912

Purpose

Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbnCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC

Plasmid

#100031

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC

Tags

1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)

Expression

Mammalian

Promoter

Adenovirus major late promoter

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX 4T1 hBad (1-146)

Plasmid

#35569

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

bad (BAD Human)

Tags

Flag

Expression

Bacterial

Mutation

residues 1-146

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only
pKS-DTA miR-106b~25

Plasmid

#19030

Depositor

Tyler Jacks

Article

Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

Insert

microRNA 106a-25 (Mir25 Mouse)

Use

Mouse Targeting

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

no stop codon

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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RCIscript-GoldyTALEN-ponzr1L

Plasmid

#42654

Depositor

Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

ponzr1 left pTAL scaffold

Use

Mrna transcription vector

Promoter

T3

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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