We narrowed to 8,147 results for: Lif

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pGI3EM31

Plasmid

#135491

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCerulean3

Tags

mCerulean3

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-DIO-APEX2NES

Plasmid

#79907

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

APEX2NES

Use

AAV and Cre/Lox

Tags

V5

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pACS142

Plasmid

#204598

Purpose

Histone H3.3K9M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human H3.3 K9M (H3-3A Human)

Use

Lentiviral

Tags

human histone H3.3 K9M - V5

Expression

Mammalian

Mutation

H3.3 K9M

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pACS145

Plasmid

#204600

Purpose

Histone H3.3K36M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.3 K36M (H3-3A Human)

Use

Lentiviral

Tags

human histone H3.3 K36M - V5

Expression

Mammalian

Mutation

histone H3.3 K36M

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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p10xQUAS-CsChrimson

Plasmid

#163629

Purpose

10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequence

Depositor

Christopher Potter

Article

Task et al Elife. 2022 Apr 20;11. pii: 72599. doi:

Insert

CsChrimson.mVenus

Tags

mVenus

Expression

Insect

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-NINJ1

Plasmid

#201179

Purpose

Expression of NINJ1 gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Use

Retroviral

Tags

Flag

Promoter

MESV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-N2c_envA-IRES-Neo

Plasmid

#172368

Purpose

Generation of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

envA + Neo

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGal-BE3

Plasmid

#172409

Purpose

Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

Depositor

Leonid Kruglyak

Article

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

BE3

Use

CRISPR

Expression

Yeast

Promoter

GALL

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD2-ChR2-mCherry

Plasmid

#170845

Purpose

AAV vector for Cre-dependent transgene expression of ChR2-mCherry in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Liu et al Elife. 2020 Oct 9;9. pii: 55267. doi: 10

Insert

ChR2-mCherry

Use

AAV

Expression

Mammalian

Promoter

Dlx

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFsynW SYT1 reporter

Plasmid

#197275

Purpose

Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Use

Lentiviral

Tags

FLAG

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVtight-RFP-IRES-CD

Plasmid

#126678

Purpose

Doxycycline inducible expression of CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

RFP-IRES-CD (FCY1 Budding Yeast)

Promoter

tight TRE

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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P445-FBP_17

Plasmid

#139708

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag

Plasmid

#179721

Purpose

Lentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-TVA-IRES-Puro

Plasmid

#172366

Purpose

Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + Puro

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)

Plasmid

#213402

Purpose

douple floxed, myristoylation (myr) site for membrane targeting and EGFP

Depositor

Katrin Willig

Article

Wegner et al Elife. 2022 Feb 23;11:e73603. doi: 10.75

Insert

EGFP

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204971

Purpose

Soma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

raACR

Use

AAV

Tags

mCerulean3

Promoter

CaMKII

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

Plasmid

#166611

Purpose

Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)

Plasmid

#166610

Purpose

Encodes Cre-inactivated ChR2-TdTomato under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

ChR2-TdTomato

Use

AAV

Mutation

H134R

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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ABHD16A-mNG

Plasmid

#195157

Purpose

mammalian expression of ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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