We narrowed to 9,867 results for: Lif

1. pFlpStop-attB-UAS-2.1-tdTom

   Plasmid

   #88910

   Purpose

   attB FlpStop plasmid, for insertion into MiMIC site

   Depositor

   Tom Clandinin

   Article

   Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

   Insert

   FlpStop
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   5XUAS

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. DNA-PKcs N-terminal sgRNA

   Plasmid

   #207093

   Purpose

   pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   AGCGGGACTCGGCGGCATGG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Halo-DNAPKcs HRD

   Plasmid

   #207088

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRXIP-GFP-P4M-SidMx2

   Plasmid

   #221747

   Purpose

   Stable expression of GFP-P4M-SidMx2 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   P4M-SidM(546-647)x2
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH7m34GW-35S:SNACS

   Plasmid

   #203576

   Purpose

   Plant SnRK2 kinase activity FRET sensor in plant expression vector

   Depositor

   Julian Schroeder

   Article

   Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

   Insert

   SNACS
   

   Use

   Tags

   StrepII

   Expression

   Plant

   Mutation

   none

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMHE-H2B-mEGFP

   Plasmid

   #105528

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   H2B (H2BC11 Human)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   T7

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-FLAG-APEX2-TAX1BP1

   Plasmid

   #175761

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   TAX1BP1 (TAX1BP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZLCv2-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106357

   Purpose

   Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMA-SpCas9-g3

   Plasmid

   #80786

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACS141

    Plasmid

    #204597

    Purpose

    Histone H3.3-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    human histone H3.3 (H3-3A Human)
    

    Use

    Tags

    human histone H3.3 - V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Cyto-CaNAR2

    Plasmid

    #64729

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2-NES
    

    Use

    Tags

    Cerulean3, Nuclear export signal (NES), and YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. optoAKT

    Plasmid

    #207164

    Purpose

    Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway

    Depositor

    Kai Zhang

    Article

    Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554

    Insert

    CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Cyto-GCaMP3

    Plasmid

    #64853

    Purpose

    Cytosol-targeted version of the Ca2+ indicator GCaMP3.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    GCaMP3
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAS12395_PEAR-GFP_2.0-preedited

    Plasmid

    #177185

    Purpose

    PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lys-eMagB-iRFP

    Plasmid

    #162249

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    p18-eMagB-iRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEBTet_NADP-Snifit-mito

    Plasmid

    #113919

    Purpose

    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TetO2

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nck

    Plasmid

    #186133

    Purpose

    For expression of recombinant Nck in bacteria cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acid residues 1-377, with mutations: C139S,…

    Promoter

    Lac

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH2B-mNG2(11)

    Plasmid

    #206043

    Purpose

    Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    H2B gene fused to the mNG2(11) fragment
    

    Use

    Synthetic Biology

    Tags

    H2B-mNG2(11)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-TRE-DIO-hM3Dq-mCherry

    Plasmid

    #166599

    Purpose

    Encodes Cre-dependent hM3Dq-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-Cyto-CaNARi

    Plasmid

    #64849

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, Nuclear export signal (NES), and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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