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We narrowed to 18,510 results for: puro

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Showing: 1801 - 1820 of 18510 results

  2. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  7. Halo-MDC1 HRD

    Plasmid
    #207086
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous MDC1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human MDC1 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207086-plasmid-map-sequence-id-431126

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  8. Halo-ATM HRD

    Plasmid
    #207085
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human ATM locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    None
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207085-plasmid-map-sequence-id-431785

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  9. Halo-SHLD3 HRD

    Plasmid
    #207077
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD3 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human SHLD3 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207077-plasmid-map-sequence-id-432393

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  10. Halo-SHLD2 HRD

    Plasmid
    #207078
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    207078-plasmid-map-sequence-id-431782

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  11. Halo-LC3B HRD

    Plasmid
    #207539
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag with internal PuroR cassette flanked by human LC3B locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    207539-plasmid-map-sequence-id-429514

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  13. pSBbi-TIR1-tTA/Pur

    Plasmid
    #171683
    Purpose
    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)
    Depositor
    Randy Poon
    Article
    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.
    Inserts
    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    Expression
    Mammalian
    Promoter
    EF-1a promoter and RPBSA promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171683-plasmid-map-sequence-id-338224

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  16. XLone-BSD SPI1 P2A mCherry

    Plasmid
    #140031
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140031-plasmid-map-sequence-id-275874

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  17. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

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  18. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid
    #89993
    Purpose
    Donor template for generation of SOX9-ntdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    Use
    CRISPR
    Promoter
    none
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    89993-plasmid-map-sequence-id-180698

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  19. pTS2353_Tier3(PB)-YPet_PuroR

    Plasmid
    #169652
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169652-plasmid-map-sequence-id-366196

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Showing: 1801 - 1820 of 18510 results