We narrowed to 2,364 results for: PUC19

1. pEasyG2_nat

   Plasmid

   #184914

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEasyG3_zeo

   Plasmid

   #184917

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA

   Plasmid

   #178107

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 + MTOR nick exon-53 sgRNAs
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJBL729

   Plasmid

   #140399

   Purpose

   Expresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-cadO-3WJdB-T
   

   Use

   Synthetic Biology

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJBL718

   Plasmid

   #140388

   Purpose

   Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-mobO-3WJdB-T
   

   Use

   Synthetic Biology

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTW324

   Plasmid

   #115955

   Purpose

   Destination Vector

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl1 P1 End
   

   Use

   Synthetic Biology

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTW064

   Plasmid

   #115951

   Purpose

   Destination Vector

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 ORF Dest
   

   Use

   Synthetic Biology

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

   Plasmid

   #173214

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPN013

   Plasmid

   #91587

   Purpose

   Express sgRNA targeting human CSMD1

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   CSMD1 (CSMD1 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTW063

    Plasmid

    #115950

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 SGP (+/- Apt) Dest
    

    Use

    Synthetic Biology

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAP2050-2

    Plasmid

    #105243

    Purpose

    mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    GFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJBL712

    Plasmid

    #140382

    Purpose

    Expresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-otrO-3WJdB-T
    

    Use

    Synthetic Biology

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAP2042-1

    Plasmid

    #105244

    Purpose

    mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTW322

    Plasmid

    #115953

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P1 With 3'UTR
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTW323

    Plasmid

    #115954

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P1 No 3'UTR
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTW325

    Plasmid

    #115956

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P2 With 3'UTR
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTW326

    Plasmid

    #115957

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P2 No 3'UTR
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTW327

    Plasmid

    #115958

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P2 End
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTW328

    Plasmid

    #115959

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P3 With E1-3'UTR
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN177

    Plasmid

    #91628

    Purpose

    Express sgRNA targeting human IDO2

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    IDO2 (IDO2 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge