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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 73,902 results for: cloning

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Showing: 1821 - 1840 of 73902 results

  1. pALuWBB

    Plasmid
    #183202
    Purpose
    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA
    Depositor
    Mitch McVey
    Insert
    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    Use
    CRISPR; Recombines at attp landing site in fly ge…
    Expression
    Insect
    Promoter
    U6-3
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    183202-plasmid-map-sequence-id-362157

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  5. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid
    #105448
    Purpose
    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector
    Depositor
    Hillel Adesnik
    Publication
    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:
    Has service
    AAV9
    Insert
    ChrimsonR-mRuby2-ST
    Use
    AAV
    Tags
    mRuby2 and soma targeting motif
    Mutation
    K176R in Chrimson
    Promoter
    hSyn
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105448-plasmid-map-sequence-id-448714

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  12. AAV-CamKIIa-jGCaMP8m-WPRE

    Plasmid
    #176751
    Purpose
    AAV-mediated expression of GCaMP8m under the CamKIIa promoter
    Depositor
    Loren Looger
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Promoter
    CamKIIalpha
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    176751-plasmid-map-sequence-id-349058

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  16. AAV-CamKIIa-jGCaMP8f-WPRE

    Plasmid
    #176750
    Purpose
    AAV-mediated expression of GCaMP8f under the CamKIIa promoter
    Depositor
    Loren Looger
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Promoter
    CamKIIalpha
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    176750-plasmid-map-sequence-id-408507

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  17. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

    Plasmid
    #112677
    Purpose
    An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde
    Insert
    Nuclear-localized floxed-mCherry EGFP
    Use
    AAV
    Tags
    Nuclear localization signal
    Promoter
    EF1a
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112677-plasmid-map-sequence-id-237606

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  18. pAAV-CAG-SomaGCaMP6f2

    Plasmid
    #158757
    Purpose
    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Publication
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has service
    AAV8 and AAV9
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158757-plasmid-map-sequence-id-310662

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Showing: 1821 - 1840 of 73902 results