We narrowed to 4,413 results for: Ado;

1. EF1a_MYC Thr58Ala_P2A_Hygro_Barcode

   Plasmid

   #120513

   Purpose

   Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC Thr58Ala (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Point mutation changing Threonine to Alanine at a…

   Promoter

   EF1a

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJ207

   Plasmid

   #162680

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Triple ORF LoxP ifgMosaic Donor (LH208)

   Plasmid

   #99625

   Purpose

   To clone the 3 ORFs for Cre dependent generation of ifgMosaics

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   N-PhiM
   

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3322_pETcon_SARS2_Omicron-BA2

   Plasmid

   #184409

   Purpose

   yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LoxP1-His-H2B-Cherry-2A (SO290)

   Plasmid

   #99616

   Purpose

   To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   6xHis-H2B-Cherry
   

   Use

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET16-mHP1alpha

   Plasmid

   #181910

   Purpose

   Bacterial protein expression vector for HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1

   Plasmid

   #10880

   Depositor

   Patrick Aebischer

   Article

   Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

   Insert

   H1-shSOD1 (SOD1 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

9. EF1a_MYC Ser62Ala_P2A_Hygro_Barcode

   Plasmid

   #120514

   Purpose

   Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC Ser62Ala (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Point mutation changing Serine to Alanine at amin…

   Promoter

   EF1a

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EphA2 430-976 pcDNA3.1

    Plasmid

    #102738

    Purpose

    Expression of c terminal EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

    Insert

    EphA2 N terminal (430-976aa) (Epha2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-mHP1alpha

    Plasmid

    #181911

    Purpose

    Bacterial protein expression vector for HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR

    Plasmid

    #196518

    Purpose

    Expression of Ctdnep1_D67E-GFP

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1_D67EsiR (CTDNEP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    D67E mutation (phosphastase dead). Silent mutatio…

    Promoter

    CMV promoter; T7 promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP Kind2 S159/181/666A

    Plasmid

    #105310

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch

    Plasmid

    #10881

    Depositor

    Patrick Aebischer

    Article

    Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

    Insert

    H1-shSOD1mismatch (SOD1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pLL5.0 ShCavin1-EGFP

    Plasmid

    #187253

    Purpose

    Lentiviral expression of shRNA targeting Cavin1

    Depositor

    Alpha Yap

    Article

    Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

    Insert

    ShRNA for Cavin1 (Cavin1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

    Plasmid

    #184407

    Purpose

    yeast surface display of the SARS-CoV-2 Delta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    L452R+T478K

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-VQRRA-P2A-GFP-PGK-Puro

    Plasmid

    #110864

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRT1-His-H2B-Cherry-2A (IG156)

    Plasmid

    #99622

    Purpose

    To clone gene of interest downstream of FRT1-His-H2B-Cherry-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    His-H2B-Cherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. 4xnr UAS ifgMosaic Donor vector (IG230)

    Plasmid

    #99631

    Purpose

    To clone the 3 ORFs for Gal4 dependent generation of ifgMosaics

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    N-PhiM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Triple ORF FRT ifgMosaic Donor (IG135)

    Plasmid

    #99626

    Purpose

    To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    PGK-Neo
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge