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pETCON-HB76/design_13

Plasmid

#30293

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB76

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB77/design_47

Plasmid

#30294

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB77

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB78/design_44

Plasmid

#30295

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB78

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB81/design_35

Plasmid

#30297

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB81

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB82/design_21

Plasmid

#30298

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB82

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB7/design_52

Plasmid

#30299

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB7

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB9/design_41

Plasmid

#30300

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB9

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-IgG3/design_30

Plasmid

#30302

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

IgG Fc Designed Binder IgG3

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-IgG9/design_59

Plasmid

#30303

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

IgG Fc Designed Binder IgG9

Expression

Yeast

Available Since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pDEST40-DJ1-D149A

Plasmid

#29406

Depositor

Mark Cookson

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Mutation

Asp149Ala

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pDest40 DHFR.dn-cHSF1

Plasmid

#134735

Purpose

Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Thy1.1 DEST

Plasmid

#17442

Purpose

Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-LC3B WT

Plasmid

#123114

Purpose

Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Blast DEST JNKKTRmRuby2

Plasmid

#59154

Purpose

Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDESTN-MTp-p53-Venus

Plasmid

#53736

Purpose

Expresses Venus tagged p53 under the metallothionein promoter

Depositor

Galit Lahav

Article

Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

Insert

p53 (TP53 Human)

Tags

Venus

Expression

Mammalian

Promoter

metallothionein promoter

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST ERKKTRClover

Plasmid

#59150

Purpose

Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pdest N-FLAG METTL1-WT puro

Plasmid

#223055

Purpose

METTL1-WT gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-WT (METTL1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-Y530F

Plasmid

#140318

Purpose

Expresses HA-tagged human Src-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Y530F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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