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pCS2 hSmad1-GM

Plasmid

#22992

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S210A ,T202A, S191A, S198A

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pLPC myc hPOT1 delta OB

Plasmid

#13241

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 delta OB (POT1 Human)

Use

Retroviral

Tags

Myc

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-puro/RAF1-P261A

Plasmid

#131728

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P261A

Promoter

CMV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594A

Plasmid

#131717

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594A

Promoter

CMV

Available Since

Nov. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 Smad1 EVE GM

Plasmid

#27997

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

Human Smad1 EVE Gsk3 mutant (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S463E S465E S210A T202A S191A S198A

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only
p3xFLAG CMV14 / BRAF-G469A

Plasmid

#131711

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G469A

Promoter

CMV

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2xVlk1-3xFlag

Plasmid

#172773

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

Insert

Vlk1 (pkdcc.1.S Frog)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro-NFLAG TRF1 deltaA

Plasmid

#16278

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deleted acidic domain (aa1-66)

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pWZL Hygro-NFLAG TRF1 deltaM

Plasmid

#16281

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deleted myb domain (aa379-439)

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pWZL Hygro-NFLAG TRF1 deltaA deltaM

Plasmid

#16297

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deleted acidic domain (aa1-66) and myb domain (aa…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pSEVA231-CRISPR

Plasmid

#138712

Purpose

Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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m2-26 mTRF2

Plasmid

#12300

Depositor

Titia de Lange

Article

Broccoli et al Nat Genet. 1997 Oct . 17(2):231-5.

Insert

mTRF2 (Terf2 )

Expression

Bacterial

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 xNoggin

Plasmid

#24960

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Noggin (nog.L Frog)

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594N

Plasmid

#131713

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594N

Promoter

CMV

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC FLAG hRap1 delta BR delta CT

Plasmid

#13257

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta CT (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-40 + 96-270 +331-399 and the p…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pLPC MYC hTel2

Plasmid

#22802

Depositor

Titia de Lange

Article

Takai et al Cell. 2007 Dec 28. 131(7):1248-59.

Insert

Telomere Maintenance 2 (TELO2 Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta MC

Plasmid

#13254

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta MC (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-131 + 265-399 and the point mu…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pCS2 mouse CV2 PC

Plasmid

#25776

Depositor

Edward De Robertis

Article

Coffinier et al Mech Dev. 2002 Dec;119 Suppl 1(Suppl 1):

Insert

cross veinless 2 (Bmper Mouse)

Tags

PC

Expression

Mammalian

Available Since

July 2, 2010

Availability

Academic Institutions and Nonprofits only

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Geph CR shRNA

Plasmid

#121068

Purpose

shRNA targeting the coding region of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Lunatic Fringe

Plasmid

#24962

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Lunatic Fringe (lfng Frog)

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only