We narrowed to 158,615 results for: Des

1. pAD/CMV/V5-DEST-Cdc42-2G

   Plasmid

   #68812

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Adenoviral

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST40-2XHA-Src-Y530F

   Plasmid

   #140318

   Purpose

   Expresses HA-tagged human Src-Y530F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   Y530F

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.2/V5-DEST CDC42EP1 WT

   Plasmid

   #69819

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3/TO/V5-DEST-Paxillin-EGFP

   Plasmid

   #215530

   Purpose

   Lentiviral transfer vector with EGFP-tagged paxillin

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   PXN (PXN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   CMV

   Available Since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

   Plasmid

   #118375

   Purpose

   Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   HA-BirA*

   Expression

   Mammalian

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST40-2XFL-Src-K298M

   Plasmid

   #140315

   Purpose

   Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   K298M

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-FRT/T0-GFP-BRCA1

   Plasmid

   #71116

   Purpose

   mammalian expression of GFP-BRCA1 siRNA resistant construct

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV/TO

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pR26 CAG/BFP Dest

   Plasmid

   #74282

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiPGK Puro DEST JNKKTRrsGreenF-E

   Plasmid

   #205764

   Purpose

   Expression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   JNK KTR rsGreenF-E
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCMV Puro DEST ERKKTRrsFastLime

    Plasmid

    #205765

    Purpose

    Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    ERK KTR rsFastLime
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-DEST53 PINK1 N-GFP

    Plasmid

    #13315

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. LC001: pMVP/PB/Hygro-DEST

    Plasmid

    #121876

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiPGK Puro DEST JNKKTRmCerulean3

    Plasmid

    #90232

    Purpose

    Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT/TO/FLAG/HA-DEST DICER

    Plasmid

    #19881

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Dicer 1, ribonuclease type III (DICER1 Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti X2 Hygro DEST (w17-1)

    Plasmid

    #17295

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Hygro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST mpeg1:tomato-CAAX

    Plasmid

    #135215

    Purpose

    Red tagged macrophages

    Depositor

    David Tobin

    Article

    Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

    Insert

    mpeg1 and tdTomato
    

    Use

    Zebrafish expression

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-FRT/T0-GFP-PALB2

    Plasmid

    #71113

    Purpose

    mammalian expession of GFP-PALB2

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    PALB2 (PALB2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV/TO

    Available Since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid

    #216283

    Purpose

    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SpyCatcher003-TEVs-SpyTag003DA
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LB601: pMVP/PB/eGFP-DEST

    Plasmid

    #121870

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST-FRT/TO-eGFP-REV7

    Plasmid

    #114128

    Purpose

    Expresses N-terminally tagged eGFP-REV7 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    REV7 (MAD2L2 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    Aug. 15, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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