We narrowed to 3,140 results for: cat.3

1. pBP-J23100

   Plasmid

   #72963

   Purpose

   Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23100 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBP-pET-RBS

   Plasmid

   #72981

   Purpose

   PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   pET RBS derived from pET vectors
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBP-J23108

   Plasmid

   #72964

   Purpose

   Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23108 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBP-J23114

   Plasmid

   #72965

   Purpose

   Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23114 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBP-T7_RBS

   Plasmid

   #72978

   Purpose

   T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7+RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBP-Strep(II)

   Plasmid

   #72984

   Purpose

   Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Strep(II) N-terminal tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBP-lacZ

   Plasmid

   #72948

   Purpose

   Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 0 - Bioparts
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTU2-b-RFP

   Plasmid

   #72959

   Purpose

   Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - b site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

9. double_sgRNA targeting e3 and e7

   Plasmid

   #190689

   Purpose

   sgRNAs targeting enhancer 3 and 7 of MYC separately

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNAs targeting enhancer 3 and 7 of MYC separately
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSIN-Pdha1

    Plasmid

    #175936

    Purpose

    knockout mouse Pdha1

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Pdha1 gRNA (Pdha1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIAeBlue

    Plasmid

    #185891

    Purpose

    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIEforRed

    Plasmid

    #185892

    Purpose

    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6_HEK3+1T>A_(PP7-C4-Q1)

    Plasmid

    #232437

    Purpose

    pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

    Insert

    3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

    Plasmid

    #136382

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CAG-eYFP-3x-miR204-5p-TS

    Plasmid

    #117380

    Purpose

    An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

    Plasmid

    #136381

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSIN-CD45

    Plasmid

    #175943

    Purpose

    knockout mouse CD45

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    CD45 gRNA (Ptprc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

    Plasmid

    #71214

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3B(E697A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

    Plasmid

    #71219

    Purpose

    dCas9 fused to the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3B(E697A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-MCS-shCHD5 #1

    Plasmid

    #68876

    Purpose

    CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only