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pMOD_B2403

Plasmid

#91071

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers

Use

CRISPR

Promoter

CmYLCV

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJK597

Plasmid

#71121

Purpose

Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

Depositor

T. J. Kappock

Article

Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

Insert

formyl-CoA:oxalate CoA-transferase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDL649

Plasmid

#231164

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlHAIRLESS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlHAIRLESS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDL518

Plasmid

#231160

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEE771

Plasmid

#231159

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEE485

Plasmid

#231158

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbATML1-1pro

Plasmid

#231149

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbSTM-5'UTR

Plasmid

#231148

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbSTM-5?UTR

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbSTM-5?UTR

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbATML1-2pro and NbATML1-1pro

Plasmid

#231155

Purpose

T-DNA encoding TRV2 with mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDL655

Plasmid

#231165

Purpose

T-DNA encoding gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

gRNA targeting SlPDS

Expression

Plant

Promoter

AtU6

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDL562

Plasmid

#231162

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlCHL1

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlCHL1

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pEE834

Plasmid

#231168

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hGRN2

Plasmid

#213683

Purpose

Expresses human granulin-2 with an N-terminal twin-Strep-FLAG tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

human Granulin-2

Use

AAV

Tags

twin-Strep-FLAG

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV GFP

Plasmid

#213685

Purpose

Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

Twin-Strep GFP

Use

AAV

Tags

Twin-Strep

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-1125

Plasmid

#217999

Purpose

Start-methionine

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Start-Met

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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AA335

Plasmid

#216069

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; HygroR_v1.1; Stop

Use

Fragment

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AA333

Plasmid

#216067

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; PuroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; PuroR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA371

Plasmid

#215989

Purpose

Fragmid fragment: (N' terminus) attenuate expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; u(S)ORF_v1.1; Stop; Start; HIV-NES_v1.2; HIV-NES_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA334

Plasmid

#216068

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; BlastR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; BlastR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V222A RAD23B

Plasmid

#201580

Purpose

Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V222A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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