We narrowed to 4,839 results for: pcr
-
-
MS1
Bacterial strain#61960PurposeC43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE hisG genes deletedDepositorBacterial resistanceNoneAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZC13 (pFA6a-TEV-6xGly-V5-HphMX)
Plasmid#44085DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, HphM…Available sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)
Plasmid#44082DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…Available sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)
Plasmid#44075DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, 12xHis tag, ADH1 transcription term…Available sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)
Plasmid#44087DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, 12xHis tag, ADH1 transcription term…Available sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF278 (pFA6a-PSc-6xGly-2xStrep-KanMX)
Plasmid#44088DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…Available sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔC
Bacterial strain#102258PurposeompC deletion strain for overproduction of outer membrane proteinsDepositorBacterial resistanceNoneAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX1-Tango
Plasmid#66439PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMRGPRX1 (MRGPRX1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CYSLTR2-Tango
Plasmid#66267PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCYSLTR2 (CYSLTR2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
ExpressionBacterialAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔAC
Bacterial strain#102261PurposeompA, ompC double knockout strain for overproduction of outer membrane proteinsDepositorBacterial resistanceNoneAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔAF
Bacterial strain#102262PurposeompA, ompF double knockout strain for overproduction of outer membrane proteinsDepositorBacterial resistanceNoneAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔABF
Bacterial strain#102267PurposeompA, lamB, ompF triple knockout strain for overproduction of outer membrane proteinsDepositorBacterial resistanceNoneAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW1328
Plasmid#69488PurposesgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegansDepositorInsertsgRNA(F+E) targeting pha-1
ExpressionWormPromoterR07E5.16 U6 promoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU9
Plasmid#69324PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOPDepositorInsertgSL2-NLS-GFPint-FgF
ExpressionBacterialAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-5 in pcDNAI/Amp
Plasmid#55625PurposeAn amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCer(1-158)-gamma-5 (GNG5 Human, Aequorea victoria)
TagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-5 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-04
Plasmid#36228DepositorInsertTRP1
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-TRP1Available sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-06
Plasmid#36230DepositorInsertURA3
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-URA3Available sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238)-beta-5 in pcDNAI/Amp
Plasmid#55763PurposeA carboxyl-terminal CFP fragment was fused to Gbeta-5. When co-expressed with an amino terminal CFP or YFP fragment fused to a Ggamma subunit or RGS7, a fluorescent signal is produced.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCFP(159-238)-beta-5 (GNB5 Human, Aequorea victoria)
TagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
