We narrowed to 4,802 results for: pcr

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E. coli BW25113 ΔtnaA ΔtrpR

Bacterial Strain

#205015

Purpose

Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

Depositor

Yajun Yan

Article

Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

Bacterial Resistance

None

Species

Escherichia coli

Available Since

Aug. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pBALU12

Plasmid

#69328

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOP

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

gSL2-NLS-mChOint-FgF

Expression

Bacterial

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
pXZX352

Plasmid

#169456

Purpose

MATA fragment in pTOPO vector

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

Mata locus

Expression

Bacterial

Promoter

lac

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBALU-ext

Plasmid

#69339

Purpose

It allows to extend the genomic insert in a fosmid.

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

kanamycin gene +pCC1FOS homology

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
PILAR

Plasmid

#22851

Depositor

Jose Conejo-Garcia

Article

Huarte et al Blood. 2008 Aug 15. 112(4):1259-68.

Insert

PILAR (CLEC2A Human)

Use

Cloning vector

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only
pLO99

Plasmid

#90219

Purpose

Complements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_03691

Use

Aspergillus

Promoter

pLac

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLO100

Plasmid

#90220

Purpose

Complements mutations in Aspergillus nidulans gene pabaA (AN6550) (mutants require p-aminobenzoic acid)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_06966

Use

Aspergillus

Promoter

pLac

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCY 3040-04

Plasmid

#36219

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEGFP-TRP1

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pKI-cjVASA-tdTomato

Plasmid

#160872

Purpose

Marmoset Targeting

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

Insert

tdTomato

Use

Marmoset targeting

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHLP701

Plasmid

#203743

Purpose

C-terminal tagging of target proteins with 3xV5-IAA17 degron

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

IAA17 auxin binding domain

Use

Pcr amplification template for gene tagging

Tags

3xV5

Promoter

none

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCY 3140-02

Plasmid

#36242

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEHA

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEHA-hphMX4

Available Since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBS1479-HTP-KanMX6

Plasmid

#154085

Purpose

For C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.

Depositor

Lidia Vasiljeva

Article

Kilchert et al Cell Rep. 2015 Dec 22;13(11):2504-2515.

Insert

his6-TEV-protein A-KanR

Use

Pcr-based tagging in yeast

Tags

his6-TEV-protein A

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GPR111-Tango

Plasmid

#66303

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR111 (ADGRF2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199195

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

Plasmid

#199197

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int-BlastR

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

Plasmid

#199198

Purpose

Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int-G418R

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pJUNB-donor

Plasmid

#112313

Purpose

CRISPR donor plasmid to tag human transcription factor JUNB with GFP

Depositor

Kevin White

Insert

JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)

Use

CRISPR

Mutation

hg38:19:12792992:C>G, hg38:19:12793018:T>G,…

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BL21ΔBF

Bacterial Strain

#102264

Purpose

lamB, ompF double knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
pREST-donor

Plasmid

#113807

Purpose

CRISPR donor plasmid to tag human transcription factor REST with GFP

Depositor

Kevin White

Insert

REST homology arms flanking EGFP-IRES-Neo cassette (REST Human)

Use

CRISPR

Mutation

rs4864608

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCY 3050-07

Plasmid

#36223

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Venus-Zeocin

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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