We narrowed to 49,311 results for: Eng

1. pBTK229

   Plasmid

   #110591

   Purpose

   BTK Type 3 coding sequence for golden gate assembly and homologous recombination

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Kanamycin resistance
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pBTK570

   Plasmid

   #110615

   Purpose

   BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   E2-Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MSP977

   Plasmid

   #65774

   Purpose

   Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135E mutation in Cas9

   Promoter

   CMV & T7

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBTK510

   Plasmid

   #110611

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBTK564

   Plasmid

   #110613

   Purpose

   BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Nanoluc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBTK520

   Plasmid

   #110604

   Purpose

   BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Ensconsin-18-mEos2

   Plasmid

   #99230

   Purpose

   Mammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   mEos2
   

   Use

   Tags

   Ensconsin (MAP7)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMV-HIS3

   Plasmid

   #65333

   Purpose

   standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   HIS3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. POT2-RFP

   Plasmid

   #65318

   Purpose

   receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSUPERIORpuro-shGAS5

    Plasmid

    #46370

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

    Insert

    GAS5 (GAS5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pBTK599

    Plasmid

    #110600

    Purpose

    BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP dropout
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pBTK509

    Plasmid

    #110610

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBTK121

    Plasmid

    #110581

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    PA3 Promoter with RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pTX209

    Plasmid

    #89269

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01 and OsPDS-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NG0886 pCI-TPI-WT-4H

    Plasmid

    #65801

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Use

    Tags

    4H (4 copies of short beta-globin 3'UTR for …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBTK615

    Plasmid

    #110609

    Purpose

    BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. POT4-RFP

    Plasmid

    #65320

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMV-LEU2

    Plasmid

    #65334

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    LEU2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMH-SFB-RNF169

    Plasmid

    #99392

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

    Depositor

    Michael Huen

    Article

    Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

    Insert

    RNF169 (RNF169 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBTK563

    Plasmid

    #110612

    Purpose

    BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Nanoluc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBTK124

    Plasmid

    #110583

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    CP25 promoter with lac operator and RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pBTK403

    Plasmid

    #110599

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBTK401

    Plasmid

    #110597

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBTK130

    Plasmid

    #110584

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    T7 promoter with lac operator and RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pBTK550d

    Plasmid

    #110617

    Purpose

    BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcold-6xHis-HRV3Csite-DmLoqsPA

    Plasmid

    #41093

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    Loquacious-PA (loqs Fly)
    

    Use

    Tags

    6xHis-HRV3Csite

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pET28a-MBP-(CBX1)3-eReader

    Plasmid

    #135090

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered x3
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUHG10.3-TetO-Rs1

    Plasmid

    #16313

    Depositor

    Bruce Conklin

    Article

    Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

    Insert

    h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Added FLAG tag; Changed Aspartic Acid 100 to Alan…

    Promoter

    Available since

    Jan. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. POT10-RFP

    Plasmid

    #65326

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. POT8-RFP

    Plasmid

    #65324

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge