We narrowed to 4,319 results for: 1-Oct

1. pAAV-CaMKII-JEDI-1P-Kv-WPRE

   Plasmid

   #202614

   Purpose

   Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-Kv
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-tdTomato-WPRE

   Plasmid

   #202610

   Purpose

   Double floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   tdTomato
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAXEF-Rap1-E63(GTP)

   Plasmid

   #32698

   Depositor

   Vicki Boussiotis

   Article

   Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

   Insert

   RAP1 (RAP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E63: Consitutively GTP-bound

   Promoter

   EF promoter

   Available since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-SRGAP1

   Plasmid

   #187269

   Purpose

   Expression of SRGAP1-EGFP

   Depositor

   Alpha Yap

   Article

   Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

   Insert

   SRGAP1 (SRGAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATP1A1_G2_Dual_sgRNA

   Plasmid

   #173201

   Purpose

   Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIV537

   Plasmid

   #177704

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKII-JEDI-1P-WPRE

   Plasmid

   #202615

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. VP-ARF

   Plasmid

   #89122

   Purpose

   mammalian expression of p14-hARF linked to VP16 activation domain

   Depositor

   Elizabeth Wilson

   Article

   Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

   Insert

   p14-hARF (CDKN2A Human)
   

   Use

   Tags

   VP16

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. 145-pGL3ctrl-3' UTR

   Plasmid

   #28175

   Depositor

   Michael Kastan

   Article

   Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

   Insert

   Luciferase flanked by tp53 UTRs (TP53 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Note that insert length is based on sum of the 2 …

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

10. GB1-His-TET1-848-952(pL91)

    Plasmid

    #124388

    Purpose

    E.Coli produced TET1-848-952 purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-848-952 wildtype (TET1 Human)
    

    Use

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LPCX BMP-RIB Q203D

    Plasmid

    #12642

    Depositor

    Rik Derynck

    Article

    Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

    Insert

    BMP-RIB (Bmpr1b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q203D, activating mutation

    Promoter

    Available since

    Oct. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pPICZ-pre-pro-alphaf-E2-Crimson

    Plasmid

    #117658

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf-E2-Crimson
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-eGFP-SynaptoZip

    Plasmid

    #122525

    Purpose

    AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

    Depositor

    Antonio Malgaroli

    Article

    Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

    Insert

    eGFP-SynaptoZip (Vamp2 Rat)
    

    Use

    AAV

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKC158

    Plasmid

    #134242

    Purpose

    CENP-A Halo expression construct

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP-A-Halo tagging construct (CENPA Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. betaTrCP mVenus reporter

    Plasmid

    #192458

    Purpose

    Fluorescent reporter for beta-TrCP activity in human cells

    Depositor

    Steven Cappell

    Article

    Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

    Insert

    CDC25B (CDC25B Human)
    

    Use

    Lentiviral

    Tags

    SV40 NLS and mVenus

    Expression

    Mammalian

    Mutation

    aa198-338

    Promoter

    EF1alpha

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-DIO-mCherry-WPRE

    Plasmid

    #202620

    Purpose

    Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-GFAP-JEDI-1P-WPRE

    Plasmid

    #202616

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAP

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-Flag-dSpCas9

    Plasmid

    #92113

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA

    Plasmid

    #178100

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ATF4 7: 5’3’ATF4.GFP

    Plasmid

    #21854

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Vector derived viral termination and poly-adenyla…

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge