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pGL3-noSV40-EC1.45-human_min1-coelacanth_min2

Plasmid

#173971

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and coelacanth minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-chicken_min2

Plasmid

#173968

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and chicken minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#7

Plasmid

#173991

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #7 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #7

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-platypus_min2

Plasmid

#173967

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and platypus minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_3XCoordinatorMutant#5-6-7

Plasmid

#173993

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #5, #6 and #7 mutated

Use

Luciferase

Mutation

3X Coordinator motif mutation #5-6-7

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_3XCoordinatorMutant

Plasmid

#173964

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 3 mutated Coordinator motifs

Use

Luciferase

Mutation

3 mutated Coordinator motifs

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-mouse_min2

Plasmid

#173965

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-lizard_min2

Plasmid

#173969

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and lizard minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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A1-LCD Aro--

Plasmid

#169716

Purpose

Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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A1-LCD Perfect Mix

Plasmid

#169718

Purpose

Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. TYR and PHE shuffled into 5 clusters.

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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