We narrowed to 5,540 results for: KRAS

1. pMSCV-puro-NKX2-8

   Plasmid

   #75080

   Purpose

   retroviral vector holding human NKX2-8 cDNA

   Depositor

   David Mu

   Article

   Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Insert

   NKX2-8 (NKX2-8 Human)
   

   Use

   Retroviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild-type, full-length, without native 3' UTR

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA041

   Plasmid

   #215963

   Purpose

   Fragmid fragment: (Pol 2 promoter) human core (short) EF1a promoter

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   EFS_v1
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKERepPar

   Plasmid

   #211827

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-GK in PLJM60

   Plasmid

   #154207

   Purpose

   Lentiviral vector for stable expression of GK

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   GK (GK Human)
   

   Use

   Lentiviral

   Tags

   N terminal FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA294

   Plasmid

   #215968

   Purpose

   Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U1a_v1
   

   Use

   Fragment

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA332

   Plasmid

   #216053

   Purpose

   Fragmid fragment: (C' terminus) 3x NLS sequences

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   SV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN1-Lck-GCaMP3

   Plasmid

   #26974

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

   Insert

   Lck-GCaMP3
   

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Plasmid contains N-terminal 26 amino acid membran…

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-RT007-GCN4scFV-VB

   Plasmid

   #163387

   Purpose

   2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   antiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMV_AA089

   Plasmid

   #216105

   Purpose

   gRNA expression for Cas12a (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gRNA expression
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFnCas12a_NT

    Plasmid

    #169838

    Purpose

    Expresses FnCas12a and guide RNA in Bacteria

    Depositor

    John van der Oost

    Article

    Mohanraju et al CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1

    Insert

    FnCas12a
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pXynA

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TPI in pCW57_tTA_Blast

    Plasmid

    #154209

    Purpose

    Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    TPI1 (TPI1 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Promoter

    TRE repeats (dox off)

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAXEF-Rap1-E63(GTP)

    Plasmid

    #32698

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Expression

    Mammalian

    Mutation

    E63: Consitutively GTP-bound

    Promoter

    EF promoter

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT9624-BEAR-cloning

    Plasmid

    #162986

    Purpose

    BEAR target cloning plasmid with split EGFP

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS1ClacZ

    Plasmid

    #55171

    Purpose

    lacZ-reporter vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

15. 6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

    Plasmid

    #174424

    Purpose

    Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Tags

    6xHis(Thrombin)EGFP(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

    Plasmid

    #177183

    Purpose

    plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    sgRNA targeting the PEAR-GFP plasmid
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-HA-SAMTOR (D190A)

    Plasmid

    #100515

    Purpose

    Expresses HA tagged SAMTOR D190A mutant in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D190A

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172505

    Purpose

    Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_836

    Plasmid

    #216088

    Purpose

    Cas9 [Sp] knockout targeting CD55, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD55 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p-mCherry-C1-GtACR1

    Plasmid

    #85464

    Purpose

    Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct ACR1 gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+enhancer)

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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