We narrowed to 3,959 results for: LYC

1. pCEP4-myc-EphB1

   Plasmid

   #200981

   Purpose

   Mammalian expression plasmid for myc-tagged EphB1

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB1 (EPHB1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

   Plasmid

   #138525

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   Control non-PRC2 binding A-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR3-FLAG-Gephyrin GC

   Plasmid

   #68818

   Purpose

   Flag tagged mammalian expression of GC domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   NIL

   Promoter

   CMV

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMG058 MBP-GS 634-666-His

   Plasmid

   #154077

   Purpose

   Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   GYS1 (GYS1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

   Plasmid

   #138524

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   PRC2-binding G-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

   Plasmid

   #168099

   Purpose

   HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nUbc-OGA(544-706)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   OGA truncated to contain residues 544-706

   Promoter

   T7/CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRB144

   Plasmid

   #180175

   Purpose

   pCDNA3.1 Construct GluN1 A714L

   Depositor

   Richard Bergeron

   Article

   Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

   Insert

   GluN1 (Grin1 Rat)
   

   Use

   Tags

   Phluorin

   Expression

   Mammalian

   Mutation

   A714L

   Promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SNS-GFP

   Plasmid

   #163662

   Purpose

   Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TNF and luminal domain from ST6GAL1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the cytosolic tail from ST,…

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LENTICRISPR-HK2_sgRNA2

   Plasmid

   #201597

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX/PD-L1 3SA

    Plasmid

    #121467

    Purpose

    Expression of GST-human PD-L1 in E. Coli

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S176A, T180A, S184A

    Promoter

    Tac

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SFS-GFP

    Plasmid

    #163655

    Purpose

    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TFR and luminal domain from ST6GAL1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This chimera contains the cytosolic tail from ST,…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX neo ACTN1-GFP, Y246E

    Plasmid

    #138294

    Purpose

    Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP, Y246E fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    ACTN1 (ACTN1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y246E

    Promoter

    CMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-2T-TRR-C421-Y2404F

    Plasmid

    #182846

    Purpose

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR3-FLAG-Gephyrin G

    Plasmid

    #68817

    Purpose

    Geph G domain expression in mamalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NIL

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBullet-cg-c

    Plasmid

    #53070

    Purpose

    destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBullet-vac-c

    Plasmid

    #53076

    Purpose

    destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBullet-er-c

    Plasmid

    #53068

    Purpose

    destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ECFP and EYFP

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBullet-cyt-c

    Plasmid

    #53066

    Purpose

    destination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ECFP and EYFP

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BMR1_02g02691-bio-His

    Plasmid

    #108042

    Purpose

    Expresses enzymatically monobiotinylated full-length BMR1_02g02691 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

    Insert

    BMR1_02g02691
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis (C termi…

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BMR1_03g01540-bio-His

    Plasmid

    #108043

    Purpose

    Expresses enzymatically monobiotinylated full-length BMR1_03g01540 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

    Insert

    BMR1_03g01540
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis (C termi…

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge