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pKDC.154

Plasmid

#222228

Purpose

Acceptor plasmid for human retron-Eco1 variants.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Type

Empty backbone

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSB1C3-pT3-tetR-sfGFP

Plasmid

#140871

Purpose

T3 promoter driven synthesis of TetR-sfGFP

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-sfGFP expressed with T3 promoter

Use

Synthetic Biology

Tags

sfGFP

Expression

Bacterial

Promoter

T3

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDBD-R

Plasmid

#50571

Purpose

Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572

Depositor

Alexander Varshavsky

Article

Turner et al Science. 2000 Sep 22;289(5487):2117-20.

Insert

DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin

Expression

Yeast

Mutation

M1R (B-gal)

Promoter

Gal1

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
KvAvs3

Plasmid

#188792

Purpose

Avs3 from Klebsiella variicola PF-1

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KvAvs3

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pUMN105

Plasmid

#233024

Purpose

Encodes eGFP for ORBIT integration. High copy number.

Depositor

Anthony Baughn

Article

Varshney et al bioRxiv [Preprint]. 2026 Jan 22:2026.01.

Type

Empty backbone

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGLU_111

Plasmid

#225792

Purpose

RS421 library variant 13463

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

13463_[Tn5]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_75

Plasmid

#225777

Purpose

RS421 library variant 13463

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

13463_[abr]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_93

Plasmid

#225807

Purpose

RS421 library variant 13463

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

13463_[Himar]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-C-GFP

Plasmid

#222601

Purpose

Expression of MAC3-tagged GFP (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEM7072

Plasmid

#214010

Purpose

PrpsM driven expression of ssrA-tagged mNeonGreen (AAV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen-AAV

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7069

Plasmid

#214007

Purpose

PrpsM driven expression of ssrA-tagged mScarlet-I (AAV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I-AAV

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7073

Plasmid

#214011

Purpose

PrpsM driven expression of ssrA-tagged mCerulean (AAV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean-AAV

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7067

Plasmid

#214005

Purpose

PrpsM driven expression of ssrA-tagged eGFP (AAV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

eGFP-AAV

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_C_RON

Plasmid

#187783

Purpose

MAC-tagged gene expression of human RON

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

RON

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLIC.B2.MPN330

Plasmid

#11536

Depositor

Sung-Hou Kim

Article

Lemesle-Varloot et al J Steroid Biochem Mol Biol. 1992 Mar . 4

Insert

FAD synthetase

Tags

HisTag, non-cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pETM33_PEX14_Pex14

Plasmid

#178484

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PEX14_Pex14 (PEX14 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_SIN3A_PAH

Plasmid

#178486

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SIN3A_PAH (SIN3A Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-RHEB-IRES-Puro

Plasmid

#120576

Purpose

Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_C_IGF1R

Plasmid

#187770

Purpose

MAC-tagged gene expression of human IGF1R

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

IGF1R (IGF1R Human)

Expression

Mammalian

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1(R40L)-GFP

Plasmid

#51408

Purpose

Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 40 mutated to leucine

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only