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We narrowed to 2,000 results for: ogn

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Showing: 1901 - 1920 of 2000 results

  1. pSL0747 (pDonor_L-MmeI)

    Plasmid
    #130647
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (L*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATG --> TGTTGGAG
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130647-plasmid-map-sequence-id-255647

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  2. pSL0746 (pDonor_R-MmeI)

    Plasmid
    #130646
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (R*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATA --> TGTTGGAA
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130646-plasmid-map-sequence-id-255642

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  3. PET28-FnCas12a-EP16

    Plasmid
    #183636
    Purpose
    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.
    Depositor
    Yunzi Luo
    Article
    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-
    Insert
    FnCas12a-EP16
    Use
    CRISPR
    Tags
    6×His Tag
    Expression
    Bacterial
    Mutation
    N607R, K613V, N617R, K180S, K660R, D616N
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183636-plasmid-map-sequence-id-375575

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  4. 3xFN-Tel-TAL/pMXs-puro

    Plasmid
    #63590
    Purpose
    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038
    Insert
    3xFVN-Tel-TAL
    Use
    Retroviral and TALEN
    Tags
    3xFLAG-tag, V5-tag, and nuclear localization sign…
    Expression
    Mammalian
    Promoter
    LTR
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63590-plasmid-map-sequence-id-108379

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  5. pChlr-8SE

    Plasmid
    #52172
    Purpose
    Encodes module 8 with amino acids 12S-16E for recognition of nt 1G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in module 8
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pChlr-4SYR

    Plasmid
    #52158
    Purpose
    Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S, 13H to 13Y, and 16Q to 16R in…
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pChlr-5SR

    Plasmid
    #52162
    Purpose
    Encodes module 5 with amino acids 12S-16R for recognition of nt 4C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16R in Module 5
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pChlr-6SE

    Plasmid
    #52164
    Purpose
    Encodes module 6 with amino acids 12S-16E for recognition of nt 3G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 6
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pChlr-4SE

    Plasmid
    #52152
    Purpose
    Encodes module 4 with amino acids 12S-16E for recognition of nt 5G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 4
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pChlr-8SR

    Plasmid
    #52174
    Purpose
    Encodes module 8 with amino acids 12S-16R for recognition of nt 1C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 8
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pChlr-7CQ

    Plasmid
    #52167
    Purpose
    Encodes module 7 with amino acids 12C-16Q for recognition of nt 2A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12C and 16E to 16Q in Module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pChlr-7NQ

    Plasmid
    #52169
    Purpose
    Encodes module 7 with amino acids 12N-16Q for recognition of nt 2U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12N and 16E to 16Q in module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pChlr-7SYR

    Plasmid
    #52170
    Purpose
    Encodes module 7 with amino acids 12S-13Y-16R for recognition of nt 2C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 13N to 13Y and 16E to 16R in module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pChlr-4SR

    Plasmid
    #52154
    Purpose
    Encodes module 4 with amino acids 12S-16R for recognition of nt 5C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 4
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pChlr-4CYQ

    Plasmid
    #52155
    Purpose
    Encodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C and 13H to 13Y in Module 4
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pChlr-5SE

    Plasmid
    #52160
    Purpose
    Encodes module 5 with amino acids 12S-16E for recognition of nt 4G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16E in Module 5
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pChlr-6SR

    Plasmid
    #52166
    Purpose
    Encodes module 6 with amino acids 12S-16R for recognition of nt 3C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 6
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1901 - 1920 of 2000 results