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Plasmid#176246PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_crRNA NR3
Plasmid#176249PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3DepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…TagsExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R42E_B
Plasmid#145988PurposeInsect Expression of DmTral-R42EDepositorInsertDmTral-R42E (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500
Plasmid#92315PurposeExpression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL) mutant K16TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92229PurposeExpression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-E2-25K
Plasmid#63572PurposeExpression of human Ubiquitin conjugating enzyme E2-25KDepositorInsertUBE2-25K (UBA7 Human)
UseTagsGSTExpressionBacterialMutationIsoform 1 (200 resiudes)PromotertacAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC018 - LshCas13a from Leptotrichia shahii
Plasmid#91908PurposeExpresses LshCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLshCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_TIA1
Plasmid#106097PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1DepositorInsertgRNA TIA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIreSneo-FLAG/HA Ago3
Plasmid#10823DepositorInsertArgonaute 3 (AGO3 Human)
UseTagsFLAG and HAExpressionMammalianMutation1 silent mutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTC-ApoE-Tet
Plasmid#85578PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liverDepositorInsertCreER
UseCre/LoxTagsExpressionMammalianMutationPromoterApoE.HCR.hAATAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_C
Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
UseTagsExpressionBacterialMutationPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_TIA1
Plasmid#106094Purposegene expression of EGFP-TIA1DepositorInsertTIA1 (TIA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_TIA1
Plasmid#106095Purposebacterial protein expression of TIA1DepositorInsertTIA1 (TIA1 Human)
UseTags6xHIs-thrombin and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_TIAL1
Plasmid#106096PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1DepositorInsertgRNA TIAL1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Tensin1
Plasmid#105297PurposeTo study localization and function of Tensin1DepositorInsertTensin1 (TNS1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/cmycDrosha
Plasmid#10828DepositorInsertDrosha (DROSHA Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET11d-hUba2
Plasmid#53136PurposeE.coli expression of full length untagged human Uba2 (SAE2)DepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/FLAG/HA-DEST TNRC6A
Plasmid#19883DepositorInsertTrinucleotide repeat containing 6A (TNRC6A Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_TIA1
Plasmid#106093Purposegene expression of FLAGHA-TIA1DepositorInsertTIA1 (TIA1 Human)
UseTagsFlagHAExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLAG/HA-DGCR8
Plasmid#10921DepositorInsertDGCR8 (DGCR8 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only