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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,376 results for: Cre

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Showing: 1921 - 1940 of 14376 results

  1. Str-KDEL_neomycin

    Plasmid
    #65306
    Purpose
    Luminal ER hook only (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    Tags
    KDEL motif and Signal Sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    65306-plasmid-map-sequence-id-110514

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  2. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    139090-plasmid-map-sequence-id-273886

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  3. pcDNA3-mem-TurboID

    Plasmid
    #149409
    Purpose
    Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection
    Depositor
    Jonathan Long
    Publication
    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mem-TurboID
    Tags
    HA and V5
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    149409-plasmid-map-sequence-id-299724

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  8. mKate2-P2A-APEX2-2xFYVE_hrs

    Plasmid
    #67663
    Purpose
    Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Publication
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mKate2-P2A-APEX2-2xFYVE
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67663-plasmid-map-sequence-id-117188

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  15. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Publication
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  16. Str-KDEL_SBP-mCherry-Ecadherin

    Plasmid
    #65287
    Purpose
    synchronize trafficking of Ecadherin from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ecadherin
    Tags
    IL-2 Signal Sequence and Streptavidin Binding Pro…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    65287-plasmid-map-sequence-id-120702

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  18. GFP-BRD9

    Plasmid
    #65380
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRD9 (BRD9 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65380-plasmid-map-sequence-id-495936

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  20. pCMV-Sport6-CD63-pHuji

    Plasmid
    #130902
    Purpose
    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.
    Depositor
    DM Pegtel
    Publication
    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD63-pHuji (CD63 Human, synthetic)
    Tags
    pHuji
    Expression
    Mammalian
    Mutation
    pHuji inserted between Gln-36 and Leu-37 in extra…
    Promoter
    CMV
    Available since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    130902-plasmid-map-sequence-id-259045

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Showing: 1921 - 1940 of 14376 results